Research Article | Volume : 2 Issue : 5, May 2012

Evaluation of Dimethylthiazol Diphenyl Tetrazolium Bromide and Propidium Iodide Inclusion Assays for the Evaluation of Cell Viability by Flow Cytometry

Nabil Ben Salem Abid Zyed Rouis Faten Nefzi Nidhal Souelah Mahjoub Aouni   

Open Access    DOI: 10.7324/JAPS.2012.2525


The natural products are widely used in many fields as pharmaceutical, flavor, industrial, and additive compounds. In many cases, there is a need to evaluate the cytotoxic effect of these products and to determine the non cytotoxic concentration at which they can be used in a safe manner. The objective of the present study was to compare two methods of evaluation the cytotoxic effect of a plant extract. The MTT assay and the PI dye-inclusion assay were used to carry out the experiments. The results were then evaluated by calorimetric and flow cytometry assays. The obtained results showed that a clear difference between the two methods and the cell death using the MTT assay seem to be overestimated. To better evaluate the cytotoxic effect of natural product, we need to carry out several in vitro and in vivo tests before the judgment that a compound is safe to use and at which concentration.

Keyword:     MTT assay Cytotoxicity Propodium Iodide Flow cytometry natural extract.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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