Research Article | Volume: 8, Issue: 3, March, 2018

CD47 is Up-Regulated on Leukaemia Cells to Avoid Apoptosis In Vitro

Ikenna Kingsley Uchendu Oliver Chukwuma Orji Chidozie Elochukwu Agu Blessing Eluke Chekwube Tochi Faith Nwosu   

Open Access   

Published:  Mar 30, 2018

DOI: 10.7324/JAPS.2018.8314
Abstract

Treatment failure in T-cell acute lymphoblastic leukaemia (T-ALL) occurs when leukemic blasts acquire resistance to chemotherapeutic agents. Current research efforts are focused on the search for targets for the development of more effective and less toxic anti-leukemic drugs. CD47 has been suggested to be involved in chemo resistance and cell metastasis. Although several potential mechanisms were suggested to explain the therapeutic effect of CD47-targeting; the different effects by CD47 are still not well understood. In this study, we assessed the effect of doxorubicin on CD47 expression in jurkat T cells. Jurkat cells in complete medium were cultured with doxorubicin (50-1000nM) or control for 0, 24, 48 and 72 hours. Cells were stained with anti-CD47 FITC. Flow cytometry analysis was used for measurement of fluorescence intensity. Cell viability was detected using trypan blue exclusion test. Treatment with doxorubicin modulated the up-regulation of CD47 on membrane surfaces of jurkat cells with no significant killing of the cells. leukaemia cells exert their anti-apoptotic role through increased CD47 expression. CD47 is a novel functional protein in jurkat T cells with promising therapeutic potential and may provide insight for targeted therapy against T-ALL disease.


Keyword:     CD47 jurkat T cells leukaemia upregulation doxorubicin chemotherapy apoptosis flow cytometry.


Citation:

Uchendu IK, Orji OC, Agu CE, Chekwube BE, Nwosu TF. CD47 is Up-Regulated on Leukaemia Cells to Avoid Apoptosis in Vitro: Application in AST assay diagnostic kit. J App Pharm Sci, 2018; 8(03): 099-103.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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