Research Article | Volume: 8, Issue: 3, March, 2018

CD47 is Up-Regulated on Leukaemia Cells to Avoid Apoptosis In Vitro

Ikenna Kingsley Uchendu Oliver Chukwuma Orji Chidozie Elochukwu Agu Blessing Eluke Chekwube Tochi Faith Nwosu   

Open Access   

Published:  Mar 30, 2018

DOI: 10.7324/JAPS.2018.8314

Treatment failure in T-cell acute lymphoblastic leukaemia (T-ALL) occurs when leukemic blasts acquire resistance to chemotherapeutic agents. Current research efforts are focused on the search for targets for the development of more effective and less toxic anti-leukemic drugs. CD47 has been suggested to be involved in chemo resistance and cell metastasis. Although several potential mechanisms were suggested to explain the therapeutic effect of CD47-targeting; the different effects by CD47 are still not well understood. In this study, we assessed the effect of doxorubicin on CD47 expression in jurkat T cells. Jurkat cells in complete medium were cultured with doxorubicin (50-1000nM) or control for 0, 24, 48 and 72 hours. Cells were stained with anti-CD47 FITC. Flow cytometry analysis was used for measurement of fluorescence intensity. Cell viability was detected using trypan blue exclusion test. Treatment with doxorubicin modulated the up-regulation of CD47 on membrane surfaces of jurkat cells with no significant killing of the cells. leukaemia cells exert their anti-apoptotic role through increased CD47 expression. CD47 is a novel functional protein in jurkat T cells with promising therapeutic potential and may provide insight for targeted therapy against T-ALL disease.

Keyword:     CD47 jurkat T cells leukaemia upregulation doxorubicin chemotherapy apoptosis flow cytometry.


Uchendu IK, Orji OC, Agu CE, Chekwube BE, Nwosu TF. CD47 is Up-Regulated on Leukaemia Cells to Avoid Apoptosis in Vitro: Application in AST assay diagnostic kit. J App Pharm Sci, 2018; 8(03): 099-103.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text


Article Metrics
468 Views 20 Downloads 488 Total



Related Search

By author names