Cephalosporin C acylase: Important role, obstacles, and strategies to optimize expression in E. coli

Muthi’ah Rasyidah Sismindari Sismindari Purwanto Purwanto   

Open Access   

Published:  May 24, 2024

DOI: 10.7324/JAPS.2024.179954
Abstract

Cephalosporins have gained popularity due to the increasing resistance to penicillin antibiotics. Since the first discovery of cephalosporin C in 1945, the development of cephalosporin-class antibiotics has continued. Cephalosporin C was converted into a 7-aminocephalosporanic acid (7-ACA) compound which became a precursor for producing cephalosporin class antibiotics through side chain modification. The conversion of cephalosporin C to 7-ACA is more beneficial when performed enzymatically using the enzyme cephalosporin C acylase (CA) compared to chemical methods. This article will present the importance of cephalosporin CA enzymes in the development of cephalosporin antibiotics, the obstacles, and strategies to optimize the expression of these enzymes in Escherichia coli hosts.


Keyword:     Cephalosporin 7-ACA cephalosporin C acylase E. coli


Citation:

Rasyidah M, Sismindari S, Purwanto P. Cephalosporin C acylase: Important role, obstacles, and strategies to optimize expression in E. coli. J Appl Pharm Sci. 2024. Online First. http://doi.org/10.7324/JAPS.2024.179954

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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