Oral antipathogenic and antiviral properties of Boesenbergia rotunda (L.) Mansf. Extracts with panduratin A as active compound

Donruedee Sanguansermsri Phanchana Sanguansermsri Kittisak Buaban Rungtip Kawaree Ian Fraser Nalin Wongkattiya   

Open Access   

Published:  May 08, 2024

DOI: 10.7324/JAPS.2024.178663
Abstract

This study investigated the anti-oral pathogen and anti-human coronavirus properties of Boesenbergia rotunda, a traditional Thai medicinal plant used for treating oral diseases. Boesenbergia rotunda Crude extracts (CBR) and B. rotunda fractionated extracts (FBR) of B. rotunda were obtained and analyzed. Panduratin A, present in both CBR and FBR, was found to play a key role in their antimicrobial efficacy. Both the extracts of B. rotunda exhibited significant antimicrobial activity against Streptococcus mutans, Streptococcus pyogenes, Streptococcus sobrinus, and Candida albicans with minimum inhibitory concentration and minimum bactericidal concentration ratios demonstrating their effectiveness. Furthermore, both extracts showed a potent and direct inhibitory effect against human coronavirus 229E, with nearly 99.99% efficacy and a 4-log reduction in viral activity at a concentration of 1,600 μg/ml within 30 minutes. This study demonstrates the potential of B. rotunda as an antiviral and antipathogenic agent, with panduratin A as a bioactive compound. FBR is more potent than CBR against oral pathogens. These findings suggest that B. rotunda is a promising herbal medicine for treating oral diseases and viral infections.


Keyword:     Boesenbergia rotunda panduratin A anti-oral pathogens anti-human Coronavirus herbal extract


Citation:

Sanguansermsri D, Sanguansermsri P, Buaban K, Kawaree R, Fraser I, Wongkattiya N. Oral antipathogenic and antiviral properties of Boesenbergia rotunda (L.) Mansf. extracts with panduratin A as an active compound. J Appl Pharm Sci. 2024. Online First. http://doi.org/10.7324/JAPS.2024.178663

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Reference

1. Ongwisespaiboon O, Jiraungkoorskul W. Fingerroot, Boesenbergia rotunda, and its aphrodisiac activity. Pharmacogn Rev. 2017;11(21):27–30. doi: https://doi.org/10.4103/phrev.phrev_50_16

2. Metwalli KH, Khan SA, Krom BP, Jabra-Rizk MA. Streptococcus mutans, Candida albicans, and the human mouth: a sticky situation. PLoS Pathog. 2013;9(10):e1003616. doi: https://doi.org/10.1371/journal.ppat.1003616

3. Chahyadi A, Hartati R, Wirasutisna KR, Elfahmi. Boesenbergia pandurata Roxb., an Indonesian medicinal plant: phytochemistry, biological activity, plant biotechnology. Procedia Chem. 2014;13:13–37. doi: https://doi.org/10.1016/j.proche.2014.12.003

4. Cheenpracha S, Karalai C, Ponglimanont C, Subhadhirasakul S, Tewtrakul S. Anti-HIV-1 protease activity of compounds from Boesenbergia pandurata. Bioorg Med Chem. 2006;14(5):1710–4. doi: https://doi.org/10.1016/j.bmc.2005.10.019

5. Sanguansermsri D, Sanguansermsri P, Buaban K, Kawaree R, Wongkattiya N. Antimicrobial activity and time-kill kinetics of Boesenbergia rotunda essential oil and geraniol alcohol against oral bacterial pathogens. J Appl Pharm Sci. 2024;12(2). (In Press).

6. Leung NHL. Transmissibility and transmission of respiratory viruses. Nat Rev Microbiol. 2021;19(8):528–45. doi: https://doi.org/10.1038/s41579-021-00535-6

7. Atun S, Handayani S, Rakhmawati A. Potential bioactive compounds isolated from Boesenbergia rotunda as antioxidant and antimicrobial agents. Pharmacogn J. 2018;10(5):513–8. doi: https://doi.org/10.5530/pj.2018.3.84

8. Wongkattiya N, Sanguansermsri P, Assawatheptawee K, Niumsup PN, Fraser I, Sanguansermsri D. Antibacterial activity of ajwain oil on multidrug-resistant Enterobacteriaceae isolated from human faeces. Songklanakarin J Sci Technol. 2022;44(3):785–93. doi: https://doi.org/10.14456/sjst-psu.2022.105

9. Lomarat P, Phanthong P, Wongsariya K, Chomnawang MT, Bunyapraphatsara N. Bioautography-guided isolation of antibacterial compounds of essential oils from Thai spices against histamine-producing bacteria. Pak J Pharm Sci. 2013;26(3):473–7.

10. Yusuf NA, Suffian M, Annuar M, Khalid N. Existence of bioactive flavonoids in rhizomes and plant cell cultures of Boesenbergia rotunda (L.) Mansf. Kulturpfl. Aust J Crop Sci. 2013;7(5):730–4.

11. Clinical and Laboratory Standards Institute (CLSI). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. 11th ed. CLSI standard M07. Wayne, PA: CLSI; 2018.

12. ASTM International. ASTM E1052-20, Standard practice to assess the activity of microbicides against viruses in suspension. [cited 2022 Oct 26]. Available from: https://www.astm.org/Standards/E1052.htm

13. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65(1–2):55–63. doi: https://doi.org/10.1016/0022-1759(83)90303-4

14. Fukumoto L, Mazza G. Assessing antioxidant and pro-oxidant activities of phenolic compounds. J Agric Food Chem. 2000;48(7):3597–604. doi: https://doi.org/10.1021/jf000220w

15. Derakhshan Z, Ferrante M, Tadi M, Ansari F, Heydari A, Hosseini MS, et al. Antioxidant activity and total phenolic content of ethanolic extract of pomegranate peels, juice, and seeds. Food Chem Toxicol. 2018;114:108–11. doi: https://doi.org/10.1016/j.fct.2018.02.023

16. Kanjanasirirat P, Saksatu A, Manopwisedjaroen S, Munyoo B, Tuchinda P, Jearawuttanakul K, et al. High-content screening of Thai medicinal plants reveals Boesenbergia rotunda extract and its component. Sci Rep. 2020;10:19963. doi: https://doi.org/10.1038/s41598-020-77003-3

17. Mazlan RNAR, Zakaria MPM, Rukayadi Y. Antimicrobial activity of fingerroot [Boesenbergia rotunda (L.) Mansf. A.] extract against Streptococcus mutans and Streptococcus sobrinus. J Pure Appl Microbiol. 2016;10(4):2965–72.

18. Limsuwan S, Voravuthikunchai SP. Bactericidal, bacteriolytic, and antibacterial virulence activities of Boesenbergia pandurata (Roxb) Schltr extract against Streptococcus pyogenes. Trop J Pharm Res. 2013;12(6):1023–8. doi: https://doi.org/10.4314/tjpr.v12i6.23

19. Vergoten G, Bailly C. Interaction of panduratin A and derivatives with the SARS-CoV-2 main protease (mpro): a molecular docking study. J Biomol Struct Dyn. 2022;17(1):1–11. doi: https://doi.org/10.1080/07391102.2022.2112618

20. Boonserm P, Khunrae P, Sutthibutpong T. A computational study on the molecular mechanisms of panduratin A as a potential inhibitor on SARS-CoV-2 protein targets. Heliyon. 2023;9:e12780. doi: https://doi.org/10.1016/j.heliyon.2022.e12780

21. Salama SM, AlRashdi AS, Abdulla MA, Hassandarvish P, Bilgen M. Protective activity of panduratin A against Thioacetamide-induced oxidative damage: demonstration with in vitro experiments using WRL-68 liver cell line. BMC Comp Alt Med. 2013;13:279. doi: https://doi.org/10.1186/1472-6882-13-279

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