Antiphotoaging effects of liposomal encapsulated superoxide dismutase extract of Bacillus altitudinis on ultraviolet-A-irradiated New Zealand rabbits’ back skin

Nurfitriyawatie Nurfitriyawatie Ana Indrayati Rizal Maarif Rukmana Sari Haryanti   

Open Access   

Published:  Feb 25, 2023

DOI: 10.7324/JAPS.2023.113079

Photoaging can occur due to sunlight exposure that emits ultraviolet-A rays. Therefore, this study aims to assess the antiphotoaging activity of superoxide dismutase (SOD) extract of Bacillus altitudinis liposomes on rabbit back skin. The liposomes were encapsulated using the film method. The antiphotoaging test was carried out on the back skin of New Zealand rabbits which were separated into four groups, namely normal, negative, and positive controls, as well as liposome B. altitudinis. They were then exposed to ultraviolet-A (UV-A) light for 2 weeks. The treatment was carried out by applying liposome extract of B. altitudinis to the back skin of rabbits once a day for 28 days. Observations were made to assess the increase in the percentage of collagen, elasticity, and moisture content. The dorsal skin of the test animals was observed histologically using van Gieson staining. The results showed that rabbits exposed to UV-A light for 2 weeks experienced a decrease in collagen, elasticity, and moisture contents. Liposomes of B. altitudinis can increase the collagen level elasticity and moisture content by 6.46%, 5.10%, and 26.90%, respectively. The histological staining revealed that B. altitudinis liposomes increased collagen levels by 36.62%. These results indicate the potential value of B. altitudinis liposomes as photoaging agents.

Keyword:     Antiphotoaging liposome SOD Bacillus altitudinis ultraviolet-A-irradiated


Nurfitriyawatie N, Indrayati A, Rukmana RM, Haryanti S. Antiphotoaging effects of liposomal encapsulated superoxide dismutase extract of Bacillus altitudinis on ultraviolet-A-irradiated New Zealand rabbits’ back skin. J Appl Pharm Sci, 2023.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Amaro-Ortiz A, Yan B, D'Orazio JA. Ultraviolet radiation, aging and the skin: prevention of damage by topical cAMP manipulation. Molecules, 2014; 19(5):6202-19; doi:10.3390/molecules19056202

Armaini A, Imelda I. The protective effect of Scenedesmus dimorphus polysaccharide as an antioxidant and antiaging agent on aging rat model induced by D-galactose. J Appl Pharm Sci, 2021; 11(5):54-63; doi:10.7324/JAPS.2021.110508

Biniek K, Levi K, Dauskardt RH. Solar UV radiation reduces the barrier function of human skin. Proc Natl Acad Sci U S A, 2012; 109(42):17111-6; doi:10.1073/pnas.1206851109

Boatman R, Kelsey J, Ball N. Acute toxicity classification for ethylene glycol mono-n-butyl ether under the globally harmonized system. Regul Toxicol Pharmacol, 2014; 68(1):41-50; doi:10.1016/j. yrtph.2013.11.004

Che M, Wang R, Wang H, Zheng XFS, Wood R, Brunswick N. Expanding roles of superoxide dismutases in cell regulation and cancer. Drug Discov Today, 2017; 21(1):143-9; doi:10.1016/j.drudis.2015.10.001. Expanding

Chen X, Peng LH, Chee SS, Shan YH, Liang WQ, Gao JQ. Nanoscaled pearl powder accelerates wound repair and regeneration in vitro and in vivo. Drug Dev Ind Pharm, 2019; 45(6):1009-16; doi:10.1080/0363 9045.2019.1593436

D'Orazio J, Jarrett S, Amaro-Ortiz A, Scott T. UV radiation and the skin. Int J Mol Sci, 2013; 14(6):12222-48; doi:10.3390/ijms140612222

Duraivel S, Shaheda SA, Basha SR, Pasha SE, Jilani S. Formulation and evaluation of Antiwrinkle activity of cream and nano emulsion of Moringa oleifera seed oil. IOSR J Pharm Biol Sci, 2014; 9(4):58-73; doi:10.9790/3008-09415873

Filipczak N, Pan J, Yalamarty SSK, Torchilin VP. Recent advancements in liposome technology. Adv Drug Deliv Rev, 2020; 156:4- 22; doi:10.1016/j.addr.2020.06.022

Guo C, Gynn M, Chang TMS. Extraction of superoxide dismutase, catalase, and carbonic anhydrase from stroma-free red blood cell hemolysate for the preparation of the nanobiotechnological complex of polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase. Artif Cells Nanomed Biotechnol, 2015; 43(3):157-62; doi:10.3109/21691401.2 015.1035479

Hartmann J, Asch F. Extraction, storage duration, and storage temperature affect the activity of ascorbate peroxidase, glutathione reductase, and superoxide dismutase in rice tissue. Biology, 2019; 8(70): 1-11.

Hou X, Qiu X, Wang Y, Song S, Cong Y, Hao J. Application and efficacy of melatonin elastic liposomes in photoaging mice. Oxid Med Cell Longev, 2022; 2022; doi:10.1155/2022/7135125

Hwang C, Yoo JT, Jung GY, Joo SH, Kim J, Cha A, Han JG, Choi NS, Kang SJ, Lee SY, Kwak SK, Song HK. Biomimetic superoxide disproportionation catalyst for anti-aging lithium-oxygen batteries [Research-article]. ACS Nano, 2019; 13(8):9190-7; doi:10.1021/ acsnano.9b03525

Ighodaro OM, Akinloye OA. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): their fundamental role in the entire antioxidant defence grid. Alexandria J Med, 2018; 54(4):287-93; doi:10.1016/j.ajme.2017.09.001

Imokawa G, Ishida K. Biological mechanisms underlying the ultraviolet radiation-induced formation of skin wrinkling and sagging I: reduced skin elasticity, highly associated with enhanced dermal elastase activity, triggers wrinkling and sagging. Int J Mol Sci, 2015; 16(4):7753- 75; doi:10.3390/ijms16047753

Islam MN, Rauf A, Fahad FI, Emran TB, Mitra S, Olatunde A, Shariati MA, Rebezov M, Rengasamy KRR, Mubarak MS. Superoxide dismutase: an updated review on its health benefits and industrial applications. Crit Rev Food Sci Nutr, 2021; 0(0):1-19; doi:10.1080/1040 8398.2021.1913400

Kang YM, Hong CH, Kang SH, Seo DS, Kim SO, Lee HY, Sim HJ, An HJ. Anti-photoaging effect of plant extract fermented with Lactobacillus buchneri on CCD-986sk fibroblasts and HaCaT keratinocytes. J Funct Biomater, 2020; 11(1):1-12; doi:10.3390/jfb11010003

Karn PR, Cho W, Park HJ, Park JS, Hwang SJ. Characterization and stability studies of a novel liposomal cyclosporin a prepared using the supercritical fluid method: comparison with the modified conventional Bangham method. Int J Nanomed, 2013; 8:365-77; doi: 10.2147/IJN.S39025

Lee MJ, Agrahari G, Kim HY, An EJ, Chun KH, Kang H, Kim YS, Bang CW, Tak LJ, Kim TY. Extracellular superoxide dismutase prevents skin aging by promoting collagen production through the activation of AMPK and Nrf2/HO-1 cascades. J Invest Dermatol, 2021; 141(10):2344- 53; doi:10.1016/j.jid.2021.02.757

Lee KO, Kim SN, Kim YC. Anti-wrinkle effects of water extracts of teas in hairless mouse. Toxicol Res, 2014; 30(4):283-9; doi:10.5487/ TR.2014.30.4.283

Leiter U, Eigentler T, Garbe C. Epidemiology of skin cancer. Adv Exp Med Biol, 2014; 810:120-40.

Manosroi A, Chutoprapat R, Abe M, Manosroi W, Manosroi J. Anti-aging efficacy of topical formulations containing niosomes entrapped with rice bran bioactive compounds. Pharm Biol, 2012; 50(2):208-24; doi: 10.3109/13880209.2011.596206

Naibaho J, Safithri M, Wijaya CH. Anti-hyperglycemic activity of encapsulated Java tea-based drink on malondialdehyde formation. J Appl Pharm Sci, 2019; 9(4):88-95; doi:10.7324/JAPS.2019.90411

Picon A, Gaya P, Medina M, Nuñez M. The effect of liposome-encapsulated Bacillus subtilis neutral proteinase on Manchego cheese ripening. J Dairy Sci, 1995; 78(6):1238-47; doi:10.3168/jds.S0022- 0302(95)76743-1

Porfire AS, Pârvu AE, Daicoviciu D, Leucu?a SE. Evaluation of antiinflamatory activity of liposome encapsulated superoxide dismutase in rats peritonitis. Farmacia, 2009; 57(4):412-23.

Rivas M, Calaf GM, Laroze D, Rojas E, Mendez J, Honeyman J, Araya MC. Solar ultraviolet A radiation and nonmelanoma skin cancer in Arica, Chile. J Photochem Photobiol B Biol, 2020; 212(October):112047; doi:10.1016/j.jphotobiol.2020.112047

Savoye I, Olsen CM, Whiteman DC, Bijon A, Wald L, Dartois L, Clavel-Chapelon F, Boutron-Ruault MC, Kvaskoff M. Patterns of ultraviolet radiation exposure and skin cancer risk: the E3N-SunExp study. J Epidemiol, 2018; 28(1):27-33; doi:10.2188/jea.JE20160166

Shanbhag S, Nayak A, Narayan R, Nayak UY. Anti-aging and sunscreens: paradigm shift in cosmetics. J Adv Pharm Bull, 2019; 9(3):348- 59; doi:10.15171/jcvtr.2015.24

Suksaeree J, Kantapak K, Sansiri P, Chatpitukpong N, Kajornwongwattana W. Skin irritation test of Thai herbal face wash gel formulations using the New Zealand white rabbits. Thai J Pharm Sci, 2018; 42(supplement):93-7. Available via proceedings/backend/proceeding_file/24_PN_FP_Sukanya Settharaksa (102-106).pdf

Sulistyoningrum E, Rosmelia R, Hamid MK, Nuraini WST. Anti-aging effects of Muntingia calabura leaves extract in D-galactose-induced skin aging mouse model. J Appl Pharm Sci, 2019; 9(9):23-9; doi:10.7324/ JAPS.2019.90904

Sun M, Ye S, Xu Z, Wan L, Zhao Y. Endophytic Bacillus altitudinis Q7 from Ginkgo biloba inhibits the growth of Alternaria alternata in vitro and its inhibition mode of action. Biotechnol Biotechnol Equip, 2021; 35(1):880-94; doi:10.1080/13102818.2021.1936639

Svobodova A, Walterova D, Vostalova J. Ultraviolet light induced alteration to the skin. Biomed Pap Med Fac Univ Palacký Olomouc Czech Repub, 2006; 150(1):25-38; doi:10.5507/bp.2006.003

Thakur A, Safety F, Chand D. Superoxide dismutase and oxidative stress: elucidation of antiaging and antioxidative effect on mammalian cell lines. J Adv Microbiol, 2017; 3:54-9.

Treiber N, Maity P, Singh K, Ferchiu F, Wlaschek M, Scharffetter- Kochanek K. The role of manganese superoxide dismutase in skin aging. Dermatoendocrinol, 2012; 4(3); doi:10.4161/derm.21819.

Tyrrell RM. Solar ultraviolet A radiation: an oxidizing skin carcinogen that activates heme oxygenase-1. Antioxid Redox Signal, 2004; 6(5):835-40; doi:10.1089/ars.2004.6.835

Vorauer-Uhl K, Fürnschlief E, Wagner A, Ferko B, Katinger H. Topically applied liposome encapsulated superoxide dismutase reduces postburn wound size and edema formation. Eur J Pharm Sci, 2001; 14(1):63-7; doi:10.1016/S0928-0987(01)00149-X

Zhou Y, Wang YP, He YH, Ding JC. Ginsenoside Rg1 performs anti-aging functions by suppressing mitochondrial pathway- mediated apoptosis and activating sirtuin 3 (SIRT3)/Superoxide dismutase 2 (SOD2) pathway in Sca-1,+ HSC/HPC cells of an aging rat model. Med Sci Monit, 2020; 26:1-8; doi:10.12659/MSM.920666

Zhuang Y, Hou H, Zhao X, Zhang Z, Li B. Effects of collagen and collagen hydrolysate from jellyfish (Rhopilema esculentum) on mice skin photoaging induced by UV irradiation. J Food Sci, 2009; 74(6); doi:10.1111/j.1750-3841.2009.01236.x

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