Exopolysaccharide from the mice ovarian bacterium Bacillus velezensis OM03 triggers caspase-3-dependent apoptosis in ovarian cancer cells

Sreejesh Pilakkavil Chirakkara; Asha Abraham

doi:10.7324/JAPS.2023.110355

Abstract

Exopolysaccharides (EPS) were isolated from mice ovarian microflora Bacillus velezensis OM03 to investigate their chemical properties and cytotoxic potential against human ovarian carcinoma cell lines PA-1 and SKOV-3. The structural analysis of EPS from OM03 using Fourier Transform Infra-Red spectroscopy, X-ray diffraction, Scanning Electron Microscopy, and Gas chromatography-Mass revealed that it is a novel heteropolysaccharide made of glucose and mannose units connected with α-1,4 and β-1,4 bonds. Attractively, the EPS inhibited the proliferation of SKOV-3 and PA-1 cancer cells in a concentration-dependent manner, with IC50 values of 620 and 238 μg/ml, respectively, according to 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tests. Moreover, EPS-OM03 caused DNA fragmentation in PA-1 cell lines and boosted the expression of active caspase-3, both of which triggered apoptosis. Furthermore, the experiment with the chick embryo chorioallantoic membrane demonstrated that treatment with EPS-OM03 exhibits an in vivo antiangiogenic effect with an IC50 of 146 μg/ml. In conclusion, our work offers scientific support for the development and use of pharmaceuticals derived from the microbiome, and it suggests that the EPS from B. velezensis OM03 may be a lead molecule in the treatment of ovarian cancer.

Keyword: Exopolysaccharides FTIR XRD GC-MS ovarian microbiome anti-ovarian cancer

Citation:

Sreejesh PC, Abraham A. Exopolysaccharide from the mice ovarian bacterium Bacillus velezensis OM03 triggers caspase- 3-dependent apoptosis in ovarian cancer cells. J Appl Pharm Sci, 2023. https://doi.org/10.7324/JAPS.2023.110355

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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