Combretum hypopilinum exhibited anti-inflammatory activity in LPS-stimulated RAW 264.7 murine macrophages through inhibition of inflammatory mediators and apoptosis

Kokou Idoh Kossivi Dosseh Mrinmoy Gautam Tchazou Kpatcha Natarajan Manikandan Amegnona Agbonon Muthiah Ramanathan Messanvi Gbeassor   

Open Access   

Published:  Jan 19, 2023

DOI: 10.7324/JAPS.2023.119177

This study has investigated the effects of Combretum hypopilinum on lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 cells. Dried root bark of C. hypopilinum was extracted with dichloromethane, ethyl acetate, and ethanol to give three extracts. To select the most protective extract, RAW 264.7 cells were treated with each extract (10, 20, 40, and 80 μg/ml) in the presence or absence of LPS (1 μg/ml) and incubated for further 24 hours. To investigate the anti-inflammatory effect of ethyl acetate extract (EaE) which was the most protective extract, RAW 264.7 cells were treated with EaE (10, 20, 40, and 80 μg/ml) 1 hour before activation with LPS (1 μg/ml) and incubated for further 24 hours. Effects of EaE on the production of inflammatory mediators [tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, IL-10, and nitric oxide (NO)] and activities of caspase-3, caspase-8, and caspase-9 were measured. Results showed that LPS induced overproduction of pro-inflammatory cytokines and NO. However, treatment with EaE increased the production of IL-10 and decreased the production of TNF-α, IL-1β, and IL-6 and the release of NO. Moreover, EaE decreased activities of caspase-3 and caspase-8. Our results indicate that C. hypopilinum inhibited LPS-induced apoptosis and inflammation through inhibition of the production of inflammatory cytokines and mediators and inhibition of apoptosis triggered by LPS.

Keyword:     Combretum hypopilinum inflammatory cytokines inflammation lipopolyssacharide apoptosis RAW 264.7 cells


Idoh K, Dosseh K, Gautam M, Kpatcha T, Manikandan N, Agbonon A, Ramanathan M, Gbeassor M. Combretum hy­popilinum exhibited anti-inflammatory activity in LPS-stim­ulated RAW 264.7 murine macrophages through inhibition of inflammatory mediators and apoptosis. J Appl Pharm Sci, 2023.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text


Adjanohoun EJ, Ahyi AMR, Ake AL, Akpagana K, Chibon P, El-Hadji A, Eyme J, Garba M, Gassita JN, Gbeassor M, Goudote E, Guinko S. Contribution aux études ethnobotaniques et floristiques au Togo. In Médecine traditionnelle et pharmacopée. Agence de Coopération Culturelle et Technique, Paris, France, pp 526-31, 1986.

Anderson D, Howlett J, McNab C. Amino acid composition of gum exudates from some African Combretum, Terminalia and Anogeissus species. Phytochemistry, 1987; 26(3):837-9.

Burkill H. The useful plants of West Tropical Africa. Royal Botanic Gardens, Kew, Richmond, UK, p 960, 1985.

Domitrovic R, Jakovac H, Blagojevic G. Hepatoprotective activity of berberine is mediated by inhibition of TNF-alpha, COX-2, and iNOS expression in CCl(4)-intoxicated mice. Toxicology, 2012; 280:33-43.

Eloff J. The antibacterial activity of 27 southern African members of the Combretaceae. South Afr J Sci, 2012; 95:148-52.

Fan G, Zhang Y, Jiang X, Zhu Y, Wang B, Su L, Cao W, Zhang H, Gao X. Anti-inflammatory activity of baicalein in LPS-stimulated RAW264.7 macrophages via estrogen receptor and NF-κB-dependent pathways. Inflammation, 2013; 36(6):1584-91.

Fyhrquist P, Mwasumbi L, Haeggstrom C, Vuorela H, Hitunen R, Vuorela P. Antifungal activity of selected species of Terminalia, Pteleopsis and Combretum (Combretaceae) collected in Tanzania. Pharm Biol, 2004; 42(4/5):308-17.

Green D, Ferguson T, Zitvogel L, Kroemer G. Immunogenic and tolerogenic cell death. Nat Rev Immunol, 2009; 9(5):353-63.

Idoh K, Dosseh K, Kpatcha T, Agbonon A, Gbeassor M. Protective effect of Combretum hypopilinum diels: root bark extract against CCl4-induced hepatotoxicity in wistar rats. Pharmacogn Res, 2018; 10(3):325-31.

Jaggi AS, Singh N. Differential effect of spironolactone in chronic constriction injury and vincristine-induced neuropathic pain in rats. Eur J Pharmacol, 2010; 648(1-3):102-9.

Liu C, Lo J, Kuo C, Chu C, Chen L, Tsai F, Tsai C, Tzang B, Kuo W, Huang C. Akt mediates 17 beta-estradiol and/or estrogen receptor-alpha inhibition of LPS-induced tumor necresis factor-alpha expression and myocardial cell apoptosis by suppressing the JNK1/2 NFkappaB pathway. J Cell Mol Med, 2009; 13(9B):3655-67.

McGaw L, Rabe T, Sparg S, Jäger, A, Eloff J, van Staden J. An investigation on the biological activity of Combretum species. J Ethnopharmacol, 2001; 75:45-50.

Moncada S, Palmer RMJ, Higgs. Nitric oxide physiology, pathophysiology and pharmacology. Pharmacol Rev, 1991; 43:109-42.

Rogers C, Coombes P. Acidic triterpene glycosides in trichome secretions differentiate subspecies of Combretum collinum in South Africa. Biochem Syst Ecol, 1999; 27:321-3.

Taylor R, Cullen S, Martin S. Apoptosis: controlled demolition at the cellular level. Nat Rev, 2008; 9(3):231-41.

Tien Y, Lin J, Lai C, Kuo C, Lin W, Tsai C, Tsai F, Cheng Y, Peng W, Huang C. Carthamus tinctorius L. prevents LPS-induced TNF alpha signaling activation and cell apoptosis through JNK1/2-NFkappaB pathway inhibition in H9c2 cardiomyoblast cells. J Ethnopharmacol, 2010; 130(3):505-13.

Xiong H, Cheng Y, Zhang X, Zhang X. Effects of taraxasterol on iNOS and COX-2 expression in LPS-induced RAW 264.7 macrophages. J Ethnopharmacol, 2014; 155:753-7.

Article Metrics

0 Absract views 2 PDF Downloads 2 Total views

   Abstract      Pdf Download

Related Search

By author names

Citiaion Alert By Google Scholar

Name Required
Email Required Invalid Email Address

Comment required