Biological potency of actinomycetes extracts from rhizosphere soil of Dacrycarpus imbricatus from Toba Samosir, North Sumatra
Published:  Jan 10, 2023DOI: 10.7324/JAPS.2023.106853
Plants, including Dacrycarpus imbricatus, have important ecological functions as a carbon source, support the soil microbial community, improve soil nutrients, and conserve water. Moreover, actinomycetes in rhizospheric soil have a high potential in producing bioactive compounds, including anti-bacterial and antioxidant compounds. This study investigated 11 actinomycetes extracts for their anti-bacterial activity against Escherichia coli and Staphylococcus aureus and antioxidant activity as the 2,2-diphenyl-1-picrylhydrazyl (DPPH-free radical scavenging. Actinomycetes strains were isolated from the rhizosphere soil of D. imbricatus. Anti-bacterial and DPPH scavenging activities were carried out by thin-layer chromatography-bioautography. Besides, the microdilution method was performed to determine IC50 and minimum inhibitory concentration values. Potential strains were identified based on molecular identification and tested for their anti-bacterial activity against Mycobacterium smegmatis. Results revealed that the highest anti-bacterial activity exhibited by two actinomycetes extracts, Streptomyces avermitilis A18TE-8 and Micromonospora terminaliae A18TE1-1, had moderate-strong anti-bacterial activity against S. aureus (8 and 128 μg/ml, respectively) and the percentage of growth inhibition against M. smegmatis was moderate (80% and 60%, respectively). Two extracts of actinomycetes M. terminaliae A18TE1-1 and Streptomyces nigrescens A18TE1-9 had a moderate antioxidant activity with IC50 values 122.96 and 98.79 μg/ml, respectively (or AAI values 0.25 and 0.31, respectively). Therefore, actinomycetes extracts from D. imbricatus rhizosphere soil could be anti-bacterial sources, especially against S. aureus and M. smegmatis, rather than antioxidant sources.
Praptiwi P, Fathoni A, Putri AL, Wulansari D, Agusta A. Biological potency of actinomycetes extracts from rhizosphere soil of Dacrycarpus imbricatus from Toba Samosir, North Sumatra. J Appl Pharm Sci, 2023. https://doi.org/10.7324/JAPS.2023.106853
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