The potential of Clausena lansium (Lour.) peel extract consumption on hyperglycemia, hyperlipidemia and augmented oxidative stress in type-2 diabetic model rats

Wathita Phachonpai; Prathakphong Riyamongkol; Dej Mann; Terdthai Tongun

doi:10.7324/JAPS.2023.112499

Abstract

Epidemiological studies have shown that the consumption of some plant components can combat type 2 diabetes mellitus (T2DM) and its complications through various biological properties such as antioxidant activity. The extract of wampee peel (WP) exhibits several medical properties, especially antioxidant effects. We aimed to explore the antihyperlipidemic, hypoglycemic, and antioxidant effects of WP peel extract in an experimental T2DM rat model. T2DM was induced in the experimental groups by a high-fat diet and streptozotocin injection (HFD/STZ). Rats with a fasting blood glucose level above 200 mg/dl were considered diabetic and were separated into six groups (control, diabetic, diabetic treated with WP peel extract at 200, 400, or 600 mg/kg, and diabetic treated with metformin at 250 mg/kg). After 4 weeks of treatment, blood samples were collected for analysis. HFD/STZ-induced rats exhibited hyperglycemia, dyslipidemia, increased HbA1c, and increased lipid peroxidation, but reduced serum antioxidant enzyme activities. However, these changes were attenuated in T2DM rats with the highest dose of WP peel extract. Our results provide strong evidence that WP peel extract has antihyperlipidemic, antidiabetic, and antioxidant properties and thus also suggest that WP peel extract may be useful in developing novel agents for the management of T2DM.

Keyword: Clausena lansium (Lour.) type 2 diabetes antioxidant antidiabetic activity antihyperlipidemia

Citation:

Phachonpai W, Riyamongkol P, Mann D, Tongun T. The potential of Clausena lansium (Lour.) peel extract consumption on hyperglycemia, hyperlipidemia and augmented oxidative stress in type-2 diabetic model rats. J Appl Pharm Sci, 2023. https://doi.org/10.7324/JAPS.2023.112499

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reference

Adebajo AC, Iwalewa EO, Obuotor EM, Ibikunle GF, Omisore NO, Adewunmi CO, Obaparusi OO, Klaes M, Adetogun GE, Schmidt TJ, Verspohl EJ. Pharmacological properties of the extract and some isolated compounds of Clausena lansium stem bark: anti-trichomonal, antidiabetic, anti-inflammatory, hepatoprotective and antioxidant effects. J Ethnopharmacol, 2009; 122:10–9.

Al-Ani FS, Al-Nimer MS, Ali FS. Dyslipidemia as a contributory factor in etiopathogenesis of diabetic neuropathy. Indian J Endocrinol Metab, 2011; 15:110–4.

Amitani M, Asakawa A, Amitani H, Inui A. The rolçe of leptin in the control of insulin-glucose axis. Front Neurosci, 2013; 7:51.

Barrière DA, Noll C, Roussy G, Lizotte F, Kessai A, Kirby K, Belleville K, Beaudet N, Longpré JM, Carpentier AC, Geraldes P, Sarret P. Combination of high-fat/high-fructose diet and low-dose streptozotocin to model long-term type-2 diabetes complications. Sci Rep, 2018; 8:424.

Bibak B, Khalili M, Rajaei Z, Soukhtanloo M, Hadjzadeh MA, Hayatdavoudi P. Effects of melatonin on biochemical factors and food and water consumption in diabetic rats. Adv Biomed Res, 2014; 3:173.

Bindu J, Narendhirakannan RT. Role of medicinal plants in the management of diabetes mellitus: a review. Biotech, 2019; 9:4.

Burgos-Morón E, Abad-Jiménez Z, Marañón AM, Iannantuoni F, Escribano-López I, López-Domènech S, Salom C, Jover A, Mora V, Roldan I, Solá E, Rocha M, Víctor VM. Relationship between oxidative stress, ER stress, and inflammation in type 2 diabetes: the battle continues. J Clin Med, 2019; 8:1385.

Chao J, Cheng HY, Chang ML, Huang SS, Liao JW, Cheng YC, Peng WH, Pao LH. Gallic acid ameliorated impaired lipid homeostasis in a mouse model of high-fat diet-and streptozotocin-induced NAFLD and diabetes through improvement of β-oxidation and ketogenesis. Front Pharmacol, 2021; 11:606759.

Chehade JM, Gladysz M, Mooradian AD. Dyslipidemia in type 2 diabetes: prevalence, pathophysiology, and management. Drugs, 2013; 73:327–39.

Forbes JM, Cooper ME. Mechanisms of diabetic complications. Physiol Rev, 2013; 93:137–88.

Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem, 1972; 18:499–502.

German JP, Wisse BE, Thaler JP, Oh-I S, Sarruf DA, Ogimoto K, Kaiyala KJ, Fischer JD, Matsen ME, Taborsky Jr GJ, Schwartz MW, Morton GJ. Leptin deficiency causes insulin resistance induced by uncontrolled diabetes. Diabetes, 2010; 59:1626–34.

Gheibi S, Kashfi K, Ghasemi A. A practical guide for induction of type-2 diabetes in rat: incorporating a high-fat diet and streptozotocin. Biomed Pharmacother, 2017; 95:605–13.

Han X, Tao YL, Deng YP, Yu JW, Cai J, Ren GF, Sun YN, Jiang GJ. Metformin ameliorates insulitis in STZ-induced diabetic mice. Peer J, 2017; 5:e3155.

Ighodaro OM. Molecular pathways associated with oxidative stress in diabetes mellitus. Biomed Pharmacother, 2018; 108:656–62.

Ighodaro OM, Akinloye OA. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): their fundamental role in the entire antioxidant defence grid. Alexandria J Med, 2018; 54:287–93.

Karunakaran U, Park KG. A systematic review of oxidative stress and safety of antioxidants in diabetes: focus on islets and their defense. Diabetes Metab J, 2013; 37:106–12.

Kumar H, Bhardwaj K, Sharma R, Nepovimova E, Ku?a K, Dhanjal DS, Verma R, Bhardwaj P, Sharma S, Kumar D. Fruit and vegetable peels: utilization of high value horticultural waste in novel industrial applications. Molecules, 2020; 25:2812.

Lackey DE, Lazaro RG, Li P, Johnson A, Hernandez-Carretero A, Weber N, Vorobyova I, Tsukomoto H, Osborn O. The role of dietary fat in obesity-induced insulin resistance. Am J Physiol Endocrinol Metab, 2016; 311:E989–97.

Liu YP, Guo JM, Liu YY, Hu S, Yan G, Qiang L, Fu YH. Carbazole alkaloids with potential neuroprotective activities from the fruits of Clausena lansium. J Agric Food Chem, 2019; 67:5764–71.

Liu GT, Li WX, Chen YY, Wei HL. Hepatoprotective action of nine constituents isolated from the leaves of Clausena lansium in mice. Drug Dev Res, 1996; 39:174–8.

Mehta RK, Koirala P, Mallick RL, Parajuli S, Jha R. Dyslipidemia in patients with type 2 diabetes mellitus in a tertiary care centre: a descriptive cross-sectional study. JNMA J Nepal Med Assoc, 2021; 59:305–9.

Mukherjee PK, Maiti K, Mukherjee K, Houghton PJ. Leads from Indian medicinal plants with hypoglycemic potentials. J Ethnopharmacol, 2006; 106:1–28.

Muttakin M, Zulfajri M. Antioxidant activity of Syzygium Cumini fruit peel extract for diabetes mellitus treatment in alloxan-induced diabetic rats. Res J Chem Environ, 2020; 24:9–13.

Nanasombat S, Yansodthee K, Jongjaited I. Evaluation of antidiabetic, antioxidant and other phytochemical properties of Thai fruits, vegetables and some local food plants. WJST, 2019; 16:851–66.

Nolan CJ, Prentki M. Insulin resistance and insulin hypersecretion in the metabolic syndrome and type 2 diabetes: time for a conceptual framework shift. Diab Vasc Dis Res, 2019; 16:118–27.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem, 1979; 95:351–8.

Perry RJ, Samuel VT, Petersen KF, Shulman GI. The role of hepatic lipids in hepatic insulin resistance and type 2 diabetes. Nature, 2014; 510:84–91.

Phachonpai W, Tongun T. Cognition enhancing effects of Clausena lansium (Lour.) peel extract attenuate chronic restraint stress-induced memory deficit in rats. Heliyon, 2021; 7:e07003.

Prasad K, Xie H, Hao J, Yang B, Qiu S, Wei X, Chen F, Jiang Y. Antioxidant and anticancer activities of 8-hydroxypsoralen isolated from wampee [Clausena lansium (Lour.) Skeels] peel. Food Chem, 2010; 118:62–6.

Rahimi R, Nikfar S, Larijani B, Abdollahi M. A review on the role of antioxidants in the management of diabetes and its complications. Biomed Pharmacother, 2005; 59:365–73.

Reed MJ, Meszaros K, Entes LJ, Claypool MD, Pinkett JG, Gadbois TM, Reaven GM. A new rat model of type 2 diabetes: the fat-fed, streptozotocin-treated rat. Metabolism, 2000; 49:1390–4.

Saeedi P, Petersohn I, Salpea P, Malanda B, Karuranga S, Unwin N, Colagiuri S, Guariguata L, Motala AA, Ogurtsova K, Shaw JE, Bright D, Williams R. Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: results from the International Diabetes Federation Diabetes Atlas, 9th edition. Diabetes Res Clin Pract, 2019; 157:107843.

Sahin K, Onderci M, Tuzcu M, Ustundag B, Cikim G, Ozercan IH, Sriramoju V, Juturu V, Komorowski JR. Effect of chromium on carbohydrate and lipid metabolism in a rat model of type 2 diabetes mellitus: the fat-fed, streptozotocin-treated rat. Metabolism, 2007; 56(9):1233–40.

Salwe KJ, Sachdev DO, Bahurupi Y, Kumarappan M. Evaluation of antidiabetic, hypolipedimic and antioxidant activity of hydroalcoholic extract of leaves and fruit peel of Punica granatum in male Wistar albino rats. J Nat Sci Biol Med, 2015; 6:56–62.

Schnedl WJ, Ferber S, Johnson JH, Newgard CB. STZ transport and cytotoxicity. Specific enhancement in GLUT2-expressing cells. Diabetes, 1994; 43:1326–33.

Singh A, Kukreti R, Saso L, Kukreti S. Mechanistic insight into oxidative stress-triggered signaling pathways and type 2 diabetes. Molecules, 2022; 27:950.

Srinivasan K, Viswanad B, Asrat L, Kaul CL, Ramarao P. Combination of high-fat diet-fed and low-dose streptozotocin-treated rat: a model for type 2 diabetes and pharmacological screening. Pharmacol Res, 2005; 52:313–20.

Sun C, Liu Y, Zhan L, Rayat G, Xiao J, Jiang H, Li X, Chen K. Anti-diabetic effects of natural antioxidants from fruits. Trends Food Sci Technol, 2020; 117:3–14.

Tangvarasittichai S. Oxidative stress, insulin resistance, dyslipidemia and type 2 diabetes mellitus. World J Diabetes, 2015; 6:456– 80.

Usha T, Middha SK, Narzary D, Brahma BK, Goyal AK. In silico and in vivo based scientific evaluation of traditional anti-diabetic herb Hodgsonia heteroclita. Bangladesh J Pharmacol. 2017; 12:165–6.

Xu X, Xie H, Wei X. Jasmonoid glucosides, sesquiterpenes and coumarins from the fruit of Clausena lansium. LWT Food Sci Technol, 2014; 59:65–9.

Zhu T, Zuo W, Yan J, Wen P, Pei Z, Lian H, Yang HC. Comparative assessment of the antioxidant activities among the extracts of different parts of Clausena lansium (Lour.) skeels in human gingival fibroblast cells. Evid Based Complement Alternat Med. 2020; 2020:3958098.

Zimmet P. Globalization, coca-colonization and the chronic disease epidemic: can the Doomsday scenario be averted? J Intern Med, 2000; 247:301–10.

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