Amino acid profiling in wild Chamaenerion angustifolium populations applying chemometric analysis

Kateryna Uminska Zigmantas Gudžinskas Liudas Ivanauskas Victoriya Georgiyants Alla Kozurak Maria Skibytska Olha Mykhailenko   

Open Access   

Published:  Jan 03, 2023

DOI: 10.7324/JAPS.2023.108931

Evaluation of the amino acid composition of plants is a determining parameter in assessing their potential effect as food supplements. Chamaenerion angustifolium (Onagraceae), commonly known as “fireweed,” is a traditional food and medicinal plant in Europe. Current research has focused on a comparative analysis of the aerial part of 15 fireweed samples collected in Ukraine and Lithuania using the Gas chromatography–mass spectrometry (GC-MS) method. The overall amino acid composition in samples of C. angustifolium was similar. Sulfur-containing amino acids (cysteine and methionine) were absent in all samples. The alanine content in the samples was the highest, and in the samples from Kharkiv (Ukraine), Ivano-Frankivsk (Ukraine), and Plung? district (Lithuania), it ranked first among others (its content in samples was 2.350, 6.090, and 2.44 mg/g, respectively). A high amount of free amino acids was recorded in the sample from Ivano-Frankivsk (Ukraine). The results of the chemometric analysis indicated L-alanine and L-phenylalanine could be used as potential quality markers for the evaluation of the plant quality. The results indicate the potential for further pharmacological studies of fireweed raw material. Considering the content of amino acids in the aerial parts of C. angustifolium, its raw material could be used for the development of medicines and dietary supplements.

Keyword:     Amino acids Epilobium angustifolium chemometric analysis GC-MS Onagraceae


Uminska K, Gudžinskas Z, Ivanauskas L, Georgiyants V, Kozurak A, Skibytska M, Mykhailenko O. Amino acid profiling in wild Chamaenerion angustifolium populations applying chemometric analysis. J Appl Pharm Sci, 2023.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text


Abudeiyh ZH, Maksytina NP, Sereda PI, Strumenskaya EN, Bryuzgina TS. Comparative study amino acid and fatty acid composition of leaves, buds and flowers Chamaenerion angustifolium (L). Ukr Sci Med Youth J, 2010; 3-4:30-3.

Albrecht J, Sidoryk-W?grzynowicz M, Zieli?ska M, Aschner M. Roles of glutamine in neurotransmission. Neuron Glia Biol, 2010; 6:263- 76.

Averett JE, Raven PH, Becker H. The flavonoids of Onagraceae: tribe Epilobieae. Am J Bot, 1979; 66:1151-5.

Azevedo MS, Seraglio SKT, Rocha G, Balderas CB, Piovezan M, Gonzaga LV, de Barcellos Falkenberg D, Fett R, de Oliveira MAL, Costa, ACO. Free amino acid determination by GC-MS combined with a chemometric approach for geographical classification of bracatinga honeydew honey (Mimosa scabrella Bentham). Food Control, 2017; 78:383-92.

Bazylko A, Kiss A, Kowalski J. Densitometric determination of flavonoids in methanolic and aqueous extracts of Epilobii angustifolii herba by use of HPTLC. J Planar Chromat, 2007; 20:53-6.

Chahardoli A, Jalilian F, Memariani Z, Farzaei MH, Shokoohinia Y. Part III. 26. Analysis of organic acids. In S. Mohammad Nabavi, M. Saeedi, S. F. Nabavi, A. S. Silva (Eds.), Recent Advances in Natural Products Analysis,1th ed., Copyright © 2020 Elsevier Inc., 2020; 767-823. ISBN 978-0-12-816455-6.

Constantin D, Coste A, Mircea T. Epilobium sp. (Willow Herb): micropropagation and production of secondary metabolites. In: Chandra S, Lata H, Varma A. (eds.). Biotechnology for medicinal plants, Springer, Berlin, Heidelberg, Germany, pp 149-70, 2013.

Ducrey B, Marston A, Gohring S, Hartmann RW, Hostettmann K. Inhibition of 5 alpha-reductase and aromatase by the ellagitannins oenothein A and oenothein B from Epilobium species. Planta Med, 1997; 63:111-4.

Ducrey B, Wolfender JL, Marston A, Hostettmann K. Analysis of flavonol glycosides of thirteen Epilobium species (Onagraceae) by LC-UV and thermospray LC-MS. Phytochem, 1995; 38:129-37.

Feshchenko H, Oleshchuk O, Slobodianiuk L, Milian I. Study of Epilobium angustifolium L. amino acids content by HPLC method. ScienceRise Pharm Sci, 2021; 6:85-90.

Fraser CM, Chapple C. The phenylpropanoid pathway in Arabidopsis. Arabidopsis Book, 2011; 9:e0152.

Fremout W, Sanyova J, Saverwyns S, Vandenabeele P, Moens L. Identification of protein binders in works of art by high-performance liquid chromatography-diode array detector analysis of their tryptic digests. Anal Bioanal Chem, 2009; 393:1991-9.

Glen AT, Lawrie W, Mclean J, Younes MEG. Triterpenoid constituents of rose-bay willow-herb. J Chem Soc C Org, 1967; 1967:510- 5.

Granica S, Bazylko A, Kiss AK. Determination of macrocyclic ellagitannin oenothein B in plant materials by HPLC-DAD-MS: method development and validation. Phytochem Anal, 2012; 23:582-7.

Granica S, Piwowarski JP, Czerwi?ska ME, Kiss AK. Phytochemistry, pharmacology and traditional uses of different Epilobium species (Onagraceae): a review. J Ethnopharmacol, 2014; 156:316-46.

Hammer Ø, Harper DA, Ryan PD. PAST: Paleontological statistics software package for education and data analysis. Palaeontol Electron, 2001?4:1-9.

Hevesi TB. Phytochemical and in vitro biological evaluation of potentially active compounds in Epilobium species. Ph.D. Dissertation, Semmelweis University, Budapest, Hungary, 2009.

Hiermann A, Mayr K. The investigation of active compounds from Epilobium species. The occurrence of sitosterol derivatives in Epilobium angustifolium L. and Epilobium parviflorum Schreb. Sci Pharm, 1985; 53:39-44.

Hildebrandt T?M, Nesi ANB, Wagner ?L, Braun HP. Amino acid catabolism in plants. Mol Plant, 2015; 8:1563-79.

Kaškonien? V, Stankevi?ius M, Drevinskas T, Aku?eca I, Kaškonas P, Bimbirait?-Survilien? K, Maruška A, Ragažinskien? O, Kornyšova O, Briedis V, Ugenskien? R. Evaluation of phytochemical composition of fresh and dried raw material of introduced Chamerion angustifolium L. using chromatographic, spectrophotometric and chemometric techniques. Phytochem, 2015; 115:184-93.

Mykhailenko O, Gudžinskas Z, Kovalyov V, Desenko V, Ivanauskas L, Bezruk I, Georgiyants V. Effect of ecological factors on the accumulation of phenolic compounds in Iris species from Latvia, Lithuania and Ukraine. Phytochem Anal, 2020a; 31(1):1-19.

Mykhailenko O, Ivanauskas L, Bezruk I, Lesyk R, Georgiyants V. Comparative investigation of amino acids content in the dry extracts of Juno bucharica, Gladiolus hybrid Zefir, Iris hungarica, Iris variegata and Crocus sativus raw materials of Ukrainian Flora. Sci Pharm, 2020b; 88:8-18.

Näsholm Ann-Brittedfast T, Ericsson A, Nordén LG. Accumulation of amino acids in some boreal forest plants in response to increased nitrogen availability. N Phytol, 1994; 126:137-43.

Nowak A, Zagórska-Dziok M, Peru?y?ska M, Cybulska K, Kucharska E, Ossowicz-Rupniewska P, Piotrowska K, Duchnik W, Kucharski ?, Sulikowski T, Dro?dzik M, Klimowicz A. Assessment of the anti-inflammatory, antibacterial and anti-aging properties and possible use on the skin of hydrogels containing Epilobium angustifolium L. extracts. Front Pharmacol, 2022; 13:896706-13.

Nowak R, Krzaczek T. Sterole w zielu Epilobium angustifolium L. Herba Polonica, 1998; 4:297-9.

Parthasarathy A, Savka MA, Hudson AO. The synthesis and role of β-alanine in plants. Front Plant Sci, 2019; 10:921.

Polezhaeva IV, Polezhaeva NI, Menyailo LN. Amino acid and mineral compositions of the vegetative part of Chamerion angustifolium. Pharm Chem J, 2007; 41:146-8.

Santosh KC, Long L, Liu M, Zhang Q and Ruan J. Light intensity modulates the effect of phosphate limitation on carbohydrates, amino acids, and catechins in tea plants (Camellia sinensis L.). Front Plant Sci, 2021; 12:743781.

Schepetkin IA, Kirpotina LN, Jakiw L, Khlebnikov AI, Blaskovich CL, Jutila MA, Quinn MT. Immunomodulatory activity of oenothein B isolated from Epilobium angustifolium. J Immunol, 2009; 183:6754-66.

Shanaida M, Hudz N, Jasicka-Misiak I, Wieczorek PP. Polyphenols and pharmacological screening of a Monarda fistulosa L. dry extract based on a hydrodistilled residue by-product. Front Pharmacol, 2021; 12:1-10. Shanaida M, Jasicka-Misiak I, Makowicz E, Stanek N, Shanaida V, Wieczorek PP. Development of high-performance thin layer chromatography method for identification of phenolic compounds and quantification of rosmarinic acid content in some species of the Lamiaceae family. J Pharm Bioallied Sci, 2020; 12(2):139-45.

Shikov AN, Poltanov EA, Dorman HJD, Makarov VG, Tikhonov VP, Hiltunen R. Chemical composition and in vitro antioxidant evaluation of commercial water-soluble willow herb (Epilobium angustifolium L.) extracts. J Agric Food Chem, 2006; 54:3617-24.

Silva RF, Rabeschini GBP, Peinado GLR, Cosmo LG, Rezende LHG, Murayama RK, Pareja M. The ecology of plant chemistry and multi-species interactions in diversified agroecosystems. Front Plant Sci, 2018; 9:1713.

Stolarczyk M, Piwowarski JP, Granica S, Stefanska J, Naruszewicz M, Kiss AK, Extracts from Epilobium sp. herbs, their components and gutmicrobiota metabolites of Epilobium ellagitannins, urolithins, inhibithormone-dependent prostate cancercells-(LNCaP) proliferation and PSA secretion. Phytother Res, 2013; 27:1842-8.

Trumbo P, Schlicker S, Yates AA, Poos M. Food and Nutrition Board of the Institute of Medicine, The National Academies. Dietary reference intakes for energy, carbohydrate, fiber, fat, fatty acids, cholesterol, protein and amino acids. J Am Diet Assoc, 2002; 102(11):1621-30.

Vitalone A, Allkanjari O. Epilobium spp: pharmacology and phytochemistry. Phytother Res, 2018; 32:1229-40.

Vitalone A, Bordi F, Baldazzi C, Mazzanti G, Saso L, Tita B. Anti-proliferative effect on a prostatic epithelial cell line (PZ-HPV-7) by Epilobium angustifolium L. Farmaco, 2001; 56:483-9.

Volochai VI, Desenko VG, Klimovych NB, Skybitska MI, Jurenko NI, Chetvernya SO, Sklyar VG, Mykhailenko OO. Peculiarities of the growth of fireweed (Epilobium angustifolium L.) in natural populations on the territory of Ukraine [??????? ??, ??????? ??, ???????? ??, ????????? ??, ???????? ??, ???????? ??, ????? ??, ?????????? ??. ??????????? ????????? ????-??? ????????????? (Epilobium angustifolium L.) ? ????????? ?????????? ?? ????????? ???????]. Agroecol J (Biol), 2021; 1:166-72]. [In Ukrainian]. Zhou Y, Danbolt NC. Glutamate as a neurotransmitter in the healthy brain. J Neural Transm (Vienna), 2014; 121:799-817.

Article Metrics

1 Absract views 4 PDF Downloads 5 Total views

   Abstract      Pdf Download

Related Search

By author names

Citiaion Alert By Google Scholar

Name Required
Email Required Invalid Email Address

Comment required