The effect of human umbilical vein endothelial cells exosomes on the skin of intrinsic aging Wistar rats

Frieda Yanuar Nugrohoaji Dharmawan Indah Julianto Arie Kusumawardani Novan Adi Setyawan Ervina Rosmarwati Endra Yustin Ellistasari   

Open Access   

Published:  Nov 15, 2022

DOI: 10.7324/JAPS.2023.130214

Aging is a complex process influenced by intrinsic and extrinsic factors. Intrinsic aging is affected by age, genetics, and hormones. A recent study has found that human umbilical vein endothelial cells (HUVEC) exosomes can serve as a new treatment for repairing and rejuvenating skin tissue. Therefore, this research aims to determine the effect of HUVEC exosomes on increasing type I collagen deposition on the skin of intrinsic aging Wistar rats. An experimental laboratory posttest-only control group study was conducted on 30 Wistar rats. The rats were divided into a control group (Group A) and treatment groups receiving 1% HUVEC exosome (Group B) and 1.5% HUVEC exosome (Group C). Collagen deposition was measured using Masson’s trichrome staining. Statistical analysis used the Kruskal–Wallis and Mann–Whitney tests (p-value < 0.05, significant). After 4 weeks, type I collagen deposition was significantly higher in the treatment groups than in the control group (p = 0.00). The mean values of collagen deposition (%) for Group A, Group B, and Group C were 15.87, 30.71, and 40.72, respectively. The Mann–Whitney test revealed that the HUVEC exosome had a significant effect on collagen deposition. Thereby, HUVEC exosomes can increase type I collagen deposition significantly and can be considered a therapeutic option for skin rejuvenation in future studies.

Keyword:     Exosomes human umbilical vein endothelial cells intrinsic aging collagen skin


Yanuar F, Dharmawan N, Julianto I, Kusumawardani A, Setyawan NA, Rosmarwati E, Ellistasari EY. The effect of human umbilical vein endothelial cells exosomes on the skin of intrinsic aging Wistar rats. J Appl Pharm Sci, 2022.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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