Research Article | Volume: 8, Issue: 11, November, 2018

The effects of individual and combination of asiatic acid and madecassoside derived from Centella asiatica (Linn.) on the viability percentage and morphological changes of mouse macrophage cell lines (J774A.1)

Nurul Hikmah Harun Wan Amir Nizam Wan Ahmad Rapeah Suppıan   

Open Access   

Published:  Nov 30, 2018

DOI: 10.7324/JAPS.2018.81116
Abstract

Asiatic acid and madecassoside are the two pentacyclic triterpenoid compounds derived from Centella asiatica (Linn.), which believed to possess major contribution in many related pharmacological activities. This research is conducted to determine the viability and the morphological changes of macrophage cells after treating with individual and combination of the mentioned compounds by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and observing through image analyzer system, respectively. Results displayed that only AA at high doses 25 and 50 μg/ml, exhibited mild inhibition of mouse macrophage cells J774A.1 viability with only the latter dose able to alter the morphology of the cells after 24 hours of treatment. Therefore, both compounds either in sole or mix form were harmless to the cells (IC50 > 50 μg/ml). Interestingly, the combination treatment also enhanced the macrophage cell viability higher than their sole treatment in every concentration. As a consequence, the bioactive compounds at none-cytotoxic level can be applied in any in-vitro further studies such as immunomodulatory and anti-inflammatory in order to prove the local traditional claim on the herb and for future benefit in a new prospective of natural product-based drugs development.


Keyword:     Asiatic acid madecassoside Centella asiatica cytotoxic macrophage.


Citation:

Harun NH, Ahmad WANW, Suppıan R. The effects of individual and combination of asiatic acid and madecassoside derived from Centella asiatica Linn. on the viability percentage and morphological changes of mouse macrophage cell lines (J774A.1). J App Pharm Sci, 2018; 8(11): 109–115.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text

Reference

Cardoso EO, Conti BJ, Santiago KB, Conte FL, Oliveira LP, Hernandes RT, Golim MD, Sforcin JM. Phenolic compounds alone or in combination may be involved in propolis effects on human monocytes. J Pharm Pharmacol, 2017; 69(1):99–108. https://doi.org/10.1111/jphp.12660

Chandrika UG, Kumara PAASP. Gotu Kola (Centella Asiatica): nutritional properties and plausible health benefits. Adv Food Nutr Res, 2015; 76:125–57. https://doi.org/10.1016/bs.afnr.2015.08.001

Chen HY, Ye XL, Cui XL, He K, Jin YN, Chen Z, Li XG. Cytotoxicity and antihyperglycemic effect of minor constituents from rhizoma coptis in HepG2 cells. Fitoterapia, 2012; 83(1):67–73. https://doi.org/10.1016/j.fitote.2011.09.014

Duque GA, Descoteaux A. Macrophage cytokines: involvement in immunity and infectious diseases. Front Immunol, 2014; 5:1–12.

Hao C, Wu B, Hou Z, Xie Q, Liao T, Wang T, Ma D. Asiatic acid inhibits LPS-induced inflammatory response in human gingival fibroblasts. Int Immunopharmacol, 2017; 50:313–18. https://doi.org/10.1016/j.intimp.2017.07.005

Jantan I, Ahmad W, Bukhari SNA. Plant-derived immunomodulators: an insight on their preclinical evaluation and clinical trials. Front Plant Sci, 2015; 6:655. https://doi.org/10.3389/fpls.2015.00655

Jayathirtha MG, Mishra SH. Preliminary immunomodulatory activities of methanol extracts of Eclipta alba and Centella asiatica. Phytomedicine, 2004; 11(4):361–5. https://doi.org/10.1078/0944711041495236

Kavitha CV, Jain AK, Agarwal C, Pierce A, Keating A, Huber KM, Serkova NJ, Wempe MF, Agarwal R, Deep G. Asiatic acid induces endoplasmic reticulum stress and apoptotic death in glioblastoma multiforme cells both in vitro and in vivo. Mol Carcinogen, 2015; 54(11):1417–29. https://doi.org/10.1002/mc.22220

Kumar S, Shalini S, Dinesh K, Karunesh K, Renu A. Immunostimulant activity of phyllanthus reticulatus poir: a useful plant for infectious tropical diseases. Asian Pac J Trop Dis, 2014; 4:S491–95. https://doi.org/10.1016/S2222-1808(14)60496-5

Lavin Y, Arthur M, Adeeb R, Miriam M. Regulation of macrophage peripheral tissues. Nat Publ Group, 2015; 15(12):731–44.

Li Z, You K, Li J, Wang Y, Xu H, Gao B, Wang J. International immunopharmacology madecassoside suppresses proliferation and invasiveness of HGF-induced human hepatocellular carcinoma cells via. Int Immunopharmacol, 2016; 33:24–32. https://doi.org/10.1016/j.intimp.2016.01.027

Liu WH, Liu TC, Mong MC. Antibacterial effects and action modes of asiatic acid. BioMedicine, 2015; 5(3):16. https://doi.org/10.7603/s40681-015-0016-7

Lokanathan Y, Omar N, Puzi ANN, Saim A, Idrus RS. Recent updates in neuroprotective and neuroregenerative potential of Centella asiatica. Malays J Med Sci, 2016; 23(1):4–14.

Mahmood A, Tiwari AK, Sahin K, Kucuk O, Ali S. Triterpenoid saponin-rich fraction of Centella asiatica decreases IL-1β and NF-Κb and augments tissue regeneration and excision wound repair. Turk J Biol, 2016; 40:399–409. https://doi.org/10.3906/biy-1507-63

Nelson AC, Kursar TA. Interactions among plant defense compounds: a method for analysis. Chemoecology, 1999; 9(2) 81–92. https://doi.org/10.1007/s000490050037

Newton K, Dixit VM. Signaling in innate immunity and inflammation. South San Francisco, California: Cold Spring Harbor Laboratory Press, 2012; 1–19.

Oyenihi AB, Novel NC, Oluwafemi OO, Bubuya M. Centella asiatica enhances hepatic antioxidant status and regulates hepatic inflammatory cytokines in type 2 diabetic rats. Pharm Biol, 2017; 55(1):1671–78. https://doi.org/10.1080/13880209.2017.1318293

Pal RS, Pal Y. Pharmacognostic review and phytochemical screening of Centella asiatica Linn. J Med Plants Stud, 2016; 4(4): 132–35.

Palcoux JB, Niaudet P, Goumy P. Side Effects of Levamisole in Children with Nephrosis. Pediatr Nephrol, 1994; 8(2):263–4. https://doi.org/10.1007/BF00865500

Prakash V, Jaiswal N, Srivastava M. A review on medicinal properties of Centella asiatica. Asian J Pharm Clin Res, 2017; 10(10):69. https://doi.org/10.22159/ajpcr.2017.v10i10.20760

Roszer T. Understanding the Mysterious M2 Macrophage through Activation Markers and Effector Mechanisms. Mediators of Inflammation, 2015; 2015: 1–16. https://doi.org/10.1155/2015/816460

Roy DC, Barman SK, Shaik MM. Current updates on Centella asiatica: phytochemistry, pharmacology and traditional uses. Med Plant Res, 2013; 4:20–36.

Sica A, Marco E, Paola A, Chiara P. Macrophage polarization in pathology. Cell Mol Life Sci, 2015; 72(21):4111–26. https://doi.org/10.1007/s00018-015-1995-y

Tolosa L, Donato MT, Gomez-lechon MJ. Chapter 26 general cytotoxicity assessment by means of the MTT assay. Methods Mol Biol, 2015; 250:333–48. https://doi.org/10.1007/978-1-4939-2074-7_26

Vinoj G, Rashmirekha P, Avinash S, Baskaralingam V. In vitro cytotoxic effects of gold nanoparticles coated with functional acyl homoserine lactone lactonase protein from Bacillus licheniformis and their antibiofilm activity against proteus species. Antimicrob Agents Chemother, 2015; 59(2):763–71. https://doi.org/10.1128/AAC.03047-14

Wagner H, Ulrich-Merzenich G. Synergy research : approaching a new generation of phytopharmaceuticals. J Nat Remedies, 2009; 9/2: 121–41. https://doi.org/10.1016/j.phymed.2008.12.018

Weiss G, Schaible UE. Macrophage defense mechanisms against intracellular bacteria. Immunol Rev, 2015; 264(1):182–203. https://doi.org/10.1111/imr.12266

Wu T, Ji G, Wenjie G, Jing G, Xixu Z. Asiatic acid inhibits lung cancer cell growth in vitro and in vivo by destroying mitochondria. Acta Pharm Sinica, 2017; B7(1):65–72. https://doi.org/10.1016/j.apsb.2016.04.003

Yasurin P, Sriariyanun M, Phusantisampan T. Review: the bioavailability activity of Centella asiatica. Int J Appl Sci Technol, 2015; 9(1):1–9. https://doi.org/10.14416/j.ijast.2015.11.001

Zahara K, Bibi Y, Tabassum S. Clinical and therapeutic benefits of Centella asiatica. Pure Appl Biol, 2014; 3(4):152–59. https://doi.org/10.19045/bspab.2014.34004

Zhang J, Lisha A, Tingting LV, Xudong J, Fang L. Asiatic acid, a triterpene, inhibits cell proliferation through regulating the expression of focal adhesion kinase in multiple myeloma cells. Oncol Lett, 2013; 6:1762–66. https://doi.org/10.3892/ol.2013.1597

Article Metrics

644 Absract views 21 PDF Downloads 665 Total views

   Abstract      Pdf Download

Related Search

By author names

Citiaion Alert By Google Scholar

Name Required
Email Required Invalid Email Address

Comment required