Research Article | Volume: 8, Issue: 11, November, 2018

Sex differences in the effects of Auricularia auricula-judae ethanolic extracts on the life span of Drosophila melanogaster during stress and non-stress conditions

JC James N. Romero Ma. Rosario Rufina E. Ramos Ourlad Alzeus G. Tantengco Paul Mark B. Medina   

Open Access   

Published:  Nov 30, 2018

DOI: 10.7324/JAPS.2018.81113
Abstract

Antioxidant enzymes can help to increase the life span of flies by allowing the organisms to adapt during the stressful conditions. Edible mushrooms are good sources of antioxidant compounds. Auricularia auricula-judae, locally known as “tenga ng daga” is a popular edible mushroom in the Philippines. We investigated the effects of chronic supplementation of wild and cultivated A. auricula-judae crude ethanolic extracts in the life span of adult Drosophila melanogaster in the presence and absence of environmental stress such as thermal stress and starvation stress. 2,2-diphenyl-1-picrylhydrazyl assay revealed a higher antioxidant activity of cultivated A. auricula-judae crude ethanolic extract (CAEE) compared with wild A. auricula-judae crude ethanolic extract (WAEE). WAEE had no effect on the life span of female flies but it increased the life span of male flies. CAEE decreased the life span of both male and female flies. Heat and starvation stress significantly reduced the life span of flies for both sexes. Life-span reduction was more evident in the female flies. Supplementation with CAEE and WAEE showed a profound sex-specific effect on the life span of D. melanogaster under stress. Our results showed that CAEE and WAEE did not increase the life span of male flies but significantly increased the life span of female flies under starvation and heat stress.


Keyword:     Auricularia auricula-judae life span heat stress energy deprivation DPPH assay.


Citation:

Romero JJN, Ramos MRRE, Tantengco OAG, Medina PMB. Sex differences in the effects of Auricularia auriculajudae ethanolic extracts on the life span of Drosophila melanogaster during stress and non-stress conditions. J App Pharm Sci, 2018; 8(11): 087–094.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Reference

Acharya K, Yonzone P, Rai M, Acharya R. Antioxidant and nitric oxide synthase activation properties of Ganoderma applanatum. Indian J Exp Biol, 2005; 43:926–9.

Aggarwal DD. Physiological basis of starvation resistance in Drosophila leontia: analysis of sexual dimorphism. J Exp Biol, 2014; 217:1849–59. https://doi.org/10.1242/jeb.096792

Asatiani MD, Elisashvili V, Wasser SP, Reznick A, Nevo E. Antioxidant activity of submerged cultured mycelium extracts of higher Basidiomycetes mushrooms. Int J Med Mushrooms, 2007; 9:151–8. https://doi.org/10.1615/IntJMedMushr.v9.i2.50

Azizur Rahman M, Abdullah N, Aminudin N. Corroborative assessment of mushroom as the graceful ageing and lifespan promoting agent. Biointerface Res Appl Chem, 2017; 7(3):2072–84.

Boonsong S, Klaypradit W, Wilaipun P. Antioxidant activities of extracts from five edible mushrooms using different extractants. Agric Nat Resour, 2016; 50:89–97. https://doi.org/10.1016/j.anres.2015.07.002

Geiger PC, Gupte AA. Heat shock proteins are important mediators of skeletal muscle insulin sensitivity. Exerc Sport Sci Rev, 2011; 39:34–42. https://doi.org/10.1097/JES.0b013e318201f236

Goenaga J, Mensch J, Fanara JJ, Hasson E. The effect of mating on starvation resistance in natural populations of Drosophila melanogaster. Evol Ecol, 2012; 26:813–23. https://doi.org/10.1007/s10682-011-9540-4

Han SK, Lee D, Lee H, Kim D, Son HG, Yang JS, Lee SJ, Kim S. OASIS 2: online application for survival analysis 2 with features for the analysis of maximal lifespan and healthspan in aging research. Oncotarget, 2016; 7:56147–52. https://doi.org/10.18632/oncotarget.11269

Hercus MJ, Loeschcke V, Rattan SIS. Lifespan extension of Drosophila melanogaster through hormesis by repeated mild heat stress. Biogerontology, 2003; 4:149–56. https://doi.org/10.1023/A:1024197806855

Huang M, Liu J, Zhang S, Mei X, Yang X. Effects of bioactive extracts from four edible mushrooms on the lifespan of Drosophila melanogaster. Mycology, 2011; 2(1):54–8. https://doi.org/10.1080/21501203.2011.568017

Hunt PR, Son TG, Wilson MA, Yu Q, Wood WH, Becker KG, Greig NH, Mattson MP, Camandola S, Wolkow CA. Extension of lifespan in C. elegans by naphthoquinones that act through stress hormesis mechanisms. PLoS One, 2011; 6(7):e21922. https://doi.org/10.1371/journal.pone.0021922

Iliadi KG, Boulianne GL. Age-related behavioral changes in Drosophila. Ann N Y Acad Sci, 2010; 1197:9–18. https://doi.org/10.1111/j.1749-6632.2009.05372.x

Kadnikova IA, Costa R, Kalenik TK, Guruleva ON, Yanguo S. Chemical composition and nutritional value of the mushroom Auricularia auricula-judae. J Food Nutr Res, 2015; 3:478–82.

Kho YS, Vikineswary S, Abdullah N, Kuppusamy UR, Oh HI. Antioxidant capacity of fresh and processed fruit bodies and mycelium of Auricularia auricula-judae (Fr.) Quél. J Med Food, 2009; 12:167–74. https://doi.org/10.1089/jmf.2007.0568

Klepsatel P, Gáliková M, Xu Y, Kühnlein RP. Thermal stress depletes energy reserves in Drosophila. Sci Rep, 2016; 6:1–12. https://doi.org/10.1038/srep33667

Kumar KVK, Prasanna KS, Ashadevi JS. Asparagus racemosus extract increases the life span in Drosophila melanogaster. J Appl Biol Biotechnol, 2015; 3:49–55.

Le Bourg E. Oxidative stress, aging and longevity in Drosophila melanogaster. FEBS Lett, 2001; 498:183–6. https://doi.org/10.1016/S0014-5793(01)02457-7

Lin WS, Chen JY, Wang JC, Chen LY, Lin CH, Hsieh TR, Wang MF, Fu TF, Wang PY. The anti-aging effects of Ludwigia octovalvis on Drosophila melanogaster and SAMP8 mice. Age (Omaha), 2014; 36:689–703. https://doi.org/10.1007/s11357-013-9606-z

Luo Y, Chen G, Li B, Ji B, Guo Y, Tian F. Evaluation of antioxidative and hypolipidemic properties of a novel functional diet formulation of Auricularia auricula and Hawthorn. Innov Food Sci Emerg Technol, 2009; 10:215–21. https://doi.org/10.1016/j.ifset.2008.06.004

Magwere T, Chapman L, Partridge L. Sex differences in the effect of dietary restriction on life span and mortality rates in female and male Drosophila melanogaster. J Gerontol A Biol Sci Med Sci, 2004; 59:3–9. https://doi.org/10.1093/gerona/59.1.B3

Morales AE, Pérez-Jiménez A, Carmen Hidalgo M, Abellán E, Cardenete G. Oxidative stress and antioxidant defenses after prolonged starvation in Dentex dentex liver. Comp Biochem Physiol C Toxicol Pharmacol, 2004; 139:153–61. https://doi.org/10.1016/j.cca.2004.10.008

Rawal S, Singh P, Gupta A, Mohanty S. Dietary intake of curcuma longa and Emblica officinalis increases life span in Drosophila melanogaster. Biomed Res Int, 2014; 2014: Article ID 910290.

Sanchez JE, Jimenez-Perez G, Liedo P. Can consumption of antioxidant rich mushrooms extend longevity?: antioxidant activity of Pleurotus spp. and its effects on Mexican fruit flies? (Anastrepha ludens) longevity. Age (Omaha), 2015; 37:1–10. https://doi.org/10.1007/s11357-015-9847-0

Sørensen JG, Kristensen TN, Kristensen KV, Loeschcke V. Sex specific effects of heat induced hormesis in Hsf-deficient Drosophila melanogaster. Exp Gerontol, 2007; 42:1123–9. https://doi.org/10.1016/j.exger.2007.09.001

Sun Y, Yolitz J, Wang C, Spangler E, Zhan M, Zou S. Aging studies in Drosophila Melanogaster., In: Tollefsbol, T (ed.). Biological aging. Methods in molecular biology (methods and protocols). Humana Press, Totowa, NJ, pp 77–93, 2013. https://doi.org/10.1007/978-1-62703-556-9_7

Velasco AJD, Medina PMB. Crude anthocyanin extract (CAE) from ballatinao black rice does not alter longevity and increases stress susceptibility of Drosophila melanogaster. Int J Curr Res Biosci Plant Biol, 2014; 1:35–42.

Wasser SP, Weis AL. Therapeutic effects of substances occurring in higher Basidiomycetes mushrooms: a modern perspective. Crit Rev Immunol, 1999; 19:65–96.

Wong R, Piper MDW, Wertheim B, Partridge L. Quantification of food intake in Drosophila. PLoS One, 2009; 4:1–10. https://doi.org/10.1371/journal.pone.0006063

Zou Y, Hu W, Ma K, Tian M. Physicochemical properties and antioxidant activities of melanin and fractions from Auricularia auricula fruiting bodies. Food Sci Biotechnol, 2015; 24:15–21. https://doi.org/10.1007/s10068-015-0003-5

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