Short Communication | Volume: 8, Issue: 10, October, 2018

Anti-oxidant, anti-inflammatory, and anti-carcinogenic activities of araloside A from Aralia elata

Je-Hyuk Lee,  Jin-Sun Kim,  Seon-Dae Shin   
Je-Hyuk Lee1, Jin-Sun Kim2, Seon-Dae Shin1

1Department of Food and Nutrition, Kongju National University, Yesan, Korea.

2Major in Food and Nutrition, Department of Integrated Life Science and Technology, Kongju National University, Yesan, Korea.

Open Access   

Published:  Oct 31, 2018

DOI: 10.7324/JAPS.2018.81017
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Abstract

The aim of this study was to explore the anti-inflammatory and anti-cancer activities of araloside A. Araloside A had a low antioxidant activity. The decrease of 2,2-Diphenyl-1-picrylhydrazyl radicals was only about 5.2% at 200 mM of araloside A. Additionally, the araloside A (200 mM) showed approximately 11% and 9.2% decrease in NO radical chemical scavenging activity and superoxide dismutase-like activity, respectively. The estimated oxygen radical absorbance capacity and hydroxyl radical absorbance capacity values of araloside A were approximately 119.7 mM trolox equivalent/g and 0.818 mM gallic acid equivalent/g, respectively. Based on cytotoxicity results, LPS-stimulated (Lipopolysaccharide) macrophage cells were treated with 0–500 μM of araloside A, a concentration range that did not reduce the viability of macrophage cells. Araloside A inhibited the production of NO in the macrophages in a concentration-dependent manner. In particular, the 500 mM of araloside A reduced NO production to less than the basal level of NO in macrophage cells. Araloside A showed cytotoxicity against the stomach cancer cell lines, SNU-638 and AGS. Additionally, the melanoma cell line B16-F1 was susceptible to araloside A-mediated cytotoxicity. However, the araloside A did not cause cytotoxicity in the ovary cancer cell line NIH: OVCAR-3. Taken together, our results indicated that the araloside A had the anti-inflammatory activity inhibiting NO production and anti-cancer activity against SNU, AGS cancer cells, and melanoma ovarian cancer cells, in spite of its low antioxidant activity. These results provide the fundamental information for araloside A as an agent against both inflammation and cancer.


Keyword:     Anti-inflammationanticanceraraloside AAralia elata.

Citation:

Lee J-HL, Kim J-S, Shin S-D. Anti-oxidant, anti-inflammatory, and anti-carcinogenic activities of araloside A from Aralia elata. J App Pharm Sci, 2018; 8(10): 129-135.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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