Mini Review | Volume: 8, Issue: 10, October, 2018

The employment of spectroscopic techniques coupled with chemometrics for authentication analysis of halal pharmaceuticals

Abdul Rohman Nina Salamah   

Open Access   

Published:  Oct 31, 2018

DOI: 10.7324/JAPS.2018.81009

Halal authentication analysis is very important to assure that the pharmaceutical products are free from non-halal components. Among non-halal matters, pig derivatives are frequently met in the market because pig derivatives (PDs) are cheaper and easily available. Molecular spectroscopy is an ideal technique for analysis of PDs in pharmaceutical products, due to its advantages, namely rapid, non-destructive, ease in operation and minimum or no sample preparation required. However, molecular spectra are sometimes overlapping and very complex which make molecular spectroscopy lack of sensitivity. Fortunately, the development of chemometrics techniques overcome this problem. This article reviewed the development of molecular spectroscopy (ultraviolet-visible, infrared, Raman, and nuclear magnetic resonance) in combination with chemometrics techniques for spectral treatment, classification, and quantification for analysis of PDs which include lard, pork and porcine gelatine for halal authentication analysis in pharmaceutical products.

Keyword:     Molecular spectroscopypig derivativeshalal pharmaceuticalchemometrics.


Abdul Rohman, Salamah N. The employment of spectroscopic techniques coupled with chemometrics for authentication analysis of halal pharmaceuticals. J App Pharm Sci, 2018; 8(10): 063-068.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Basri KN, Hussain MN, Bakar J, Khir MFA, Zoolfakar AS, Sharif Z. Classification and quantification of palm oil adulteration via portable NIR spectroscopy. Spectrochim. Acta Part A, 2017; 173:335-342.

Behera S, Ghanty S, Ahmad F, Santra S, Banerjee S. UV-Visible Spectrophotometric Method Development and Validation of Assay of Paracetamol Tablet Formulation. J Anal Bioanal Techniques, 2012; 3:151. doi: 10.4172/2155-9872.1000151.

Bosque-Sendra JM, Cuadros-Rodríguez L, Ruiz-Samblás C, Paulina de la Mata A. Combining chromatography and chemometrics for the characterization and authentication of fats and oils from triacylglycerol compositional data-a review. Anal Chim Acta, 2012; 724:1-11.

Bro R, Jerome JW, Paul RM, Kowalski BR. Review of chemometrics applied to spectroscopy: 1985-95, Part 3—Multi-way analysis. App Spectros Rev, 1997; 32:37-261. Bumbrah GS, Sharma RM. Raman spectroscopy – Basic principle, instrumentation and selected applications for the characterization of drugs of abuse. Egyptian J Foren Sci, 2016; 6:209-215.

Cebi N, Durak MZ, Toker OS, Sagdic O, Arici M. An evaluation of Fourier transforms infrared spectroscopy method for the classification and discrimination of bovine, porcine and fish gelatins. Food Chem, 2016; 190:1109-1115.

Chen D-D, Xie X-F, Ao H, Liu J-L, Peng C. Raman spectroscopy in quality control of Chinese herbal medicine. J Chin Med Assoc, 2017; 80:288-296.

Cordella C, Moussa I, Martel AC, Sbirrazzuoli N, Cuvelier LL. Recent developments in food characterization and adulteration detection: Technique-oriented perspectives. J Agric Food Chem, 2002; 50:1751-1764.

Cozzolino D. The Role of Visible and Infrared Spectroscopy Combined with Chemometrics to Measure Phenolic Compounds in Grape and Wine Samples. Molecules, 2015; 20:726-737.

Dais P, Hatzakis E. Quality assessment and authentication of virgin olive oil by NMR spectroscopy: a critical review. Anal Chin Acta, 2013; 765:1-27.

Danezis GP, Tsagkaris AS, Camin F, Brusic V, Georgiou CA. Food authentication: Techniques, trends & emerging approaches. Trends Anal Chem, 2016; 85:123-132.

Davidson AG. 2002. Ultraviolet-visible absorption spectrophotometry. In: Beckett AH, Stenlake JB, (4th Ed.), Practical Pharmaceutical chemistry. New Delhi: CBS Publishers and distributors 275-278.

Erwanto Y, Muttaqien AT, Sugiyono, Sismindari, Rohman A. Use of Fourier transform infrared (FTIR) spectroscopy and chemometrics for analysis of lard adulteration in "rambak" crackers. Int J Food Prop, 2016; 19(12):2718-2725.

Gad HA, El-Ahmady SH, Abou-Shoer MI, Al-Azizi MM. Application of Chemometrics in Authentication of Herbal Medicines: A Review. Phytochem Anal, 2013; 24:1-24.

Gemperline P. 2006. Practical Guide to Chemometrics. Boca Raton: CRC/Taylor & Francis.

Gendrin C, Roggo Y, Collet C. Pharmaceutical applications of vibrational chemical imaging and chemometrics: A review. J Pharm Biomed Anal, 2008; 48:533-553.

Griffiths P, Chalmers JM. 2002. Handbook of vibrational spectroscopy. New York: John Wiley and Sons.

Hannah RW. 2002. Standard sampling techniques for infrared spectroscopy. In: Chalmers JM, Griffiths PR, Eds. Handbook of vibrational spectroscopy, vol. 2. London: John Wiley & Sons 933-952.

Hanrahan G, Gomez FA. 2010. Chemometric Methods in Capillary Electrophoresis. Hoboken, NJ: J. Wiley & Sons, Inc.

Hashin DM, Che Man YB, Norakasha R, Shuhaimi M, Salmah Y, Syahariza ZA. Potential use of Fourier transform infrared spectroscopy for differentiation of bovine and porcine gelatins. Food Chem, 2010; 118:856-860.

Hemmateenejad B. Chemometrics in Iran. Chem Intel Lab Sys, 2006; 81:202-208.

Kalantri PP, Somani RR, Makhija T. Raman spectroscopy: a potential technique in analysis of pharmaceuticals. Der Chem Sin, 2010; 1:1-12.

Khattak JZK, Mir A, Anwar Z, Wahedi HM, Abbas G, Khattak HZK, Ismatullah H. Concept of halal food and biotechnology. Adv J Food Sci Technol, 2011; 3(5):385-389.

Kokot S, Grigg M, Panayiotou H, Phuong TD. Data interpretation by some common chemometrics methods (tutorial review). Electroanalysis, 1998; 10(16):1081-1088.<1081::AID-ELAN1081>3.0.CO;2-X

Lubis HN, Mohd-Naim NF, Alizul NN, Ahmed MU. From market to food plate: Current trusted technology and innovations in halal food analysis. Trends Food Sci Technol, 2016; 58:55-68.

Lukitaningsih E, Saadah M, Purwanto, Rohman A. Quantitative Analysis of Lard in Lotion Cosmetics Formulation Using FTIR Spectroscopy And Partial Least Square Calibration. J Am Oil Chem Soc, 2012; 89:1537-1543.

Luypaert J, Massart DL, Vander Heyden Y. Review Near-infrared spectroscopy applications in pharmaceutical analysis. Talanta, 2007; 72:865-883.

Miller JN, Miller JC. 2010. Statistics and chemometrics for Analytical Chemistry, 5th ed. Harlow, England: Prentice Hall.

Nakyinsige K, Che Man YB, Sazili AQ. Review Halal authenticity issues in meat and meat products. Meat Sci, 2012; 91:207-214.

Norgaard L. A multivariate chemometric approach to fluorescence spectroscopy. Talanta, 1995; 42:1305-1324.

Pavia DL, Lampman GM, Kriz-jr GS. 2009. Introdoction to Specroscopy: A Guide for Students of Organic Chemistry. Philadelpia, USA: W.B. Saunders Company.

Ragno G, Ioele G, Risoli A. Multivariate calibration techniques applied to the spec-trophotometric analysis of one-to-four component systems. Anal Chimica Acta, 2004; 512:173-180.

Regenstein JM, Chaudry MM, Regenstein CE. The kosher and halal food laws. Comprehen. Rev Food Sci Food Saf, 2003; 2(3):111-127.

Reich G. Near-infrared spectroscopy and imaging: Basic principles and pharmaceutical applications. Adv Drug Deliv Rev, 2005; 57:1109-1143.

Rinnan A. Pre-processing in vibrational spectroscopy-when, why and how. Anal Methods, 2014; 6:7124-7129.

Rohman A. Application of FTIR spectroscopy for quality control in pharmaceutical products: a review. Indonesian J Pharm, 2012; 23(1):1-8.

Rohman A, Nugroho A, Lukitaningsih E, Sudjadi. Application of Vibrational Spectroscopy in Combination with Chemometrics Techniques for Authentication of Herbal Medicine. App Spectros Rev, 2014; 49:603- 613. Doi: 10.1080/05704928.2014.882347.

Rohman A, Arsyanti L, Erwanto Y, Pranoto Y. The use of vibrational spectroscopy and chemometrics in the analysis of pig derivatives for halal authentication. Int Food Res J, 2016; 23(5):1839-1848.

Rohman A. The use of infrared spectroscopy in combination with chemometrics for quality control and authentication of edible fats and oils: A review. App Spectros Rev, 2017. Doi: 10.1080/05704928.2016.1266493.

Rohman A, Che Man YB. Analysis of Lard in Cream Cosmetics Formulations using FT-IR Spectroscopy and Chemometrics. Middle-East J Sci Res, 2011; 7(5):726-732.

Rohman A, Che Man YB. Analysis of pig derivatives for halal authentication studies. Food Rev Int, 2012; 28(1):97-112.

Rohman A, Kuwat T, Retno S, Sismindari, Yuny E, Tridjoko W. Fourier Transform Infrared Spectroscopy applied for rapid analysis of lard in palm oil. Int Food Res J, 2012; 19:1161-1165.

Sádecká J, Tóthová J. Fluorescence spectroscopy and chemometrics in the food classification a review. Czech J Food Sci, 2007; 25:159-173.

Skoog DA, Holler FJ, Crouch SR. 2007. Principles of Instrumental Analysis, 6th Ed. Belmont, USA: Thomson Higher Education.

Wang P, Sun J, Zhang T, Liu W. Vibrational spectroscopic approaches for the quality evaluation and authentication of virgin olive oil. App Spectros Rev, 2016; 51:763-790.

Wartewig S, Neubert RHH. Pharmaceutical applications of Mid- IR and Raman spectroscopy. Adv Drug Deliv Rev, 2005; 57:1144-1170.

Waskito D, Lukitaningsih E, Sudjadi, Rohman A. Analysis of Lard in Lipstick Using FTIR Spectroscopy and chemometrics: A Comparison of Three Extraction Methods. J Oleo Sci, 2016; 65(10):815- 824.

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