Research Article | Volume: 8, Issue: 10, October, 2018

Oral administration of resveratrol ameliorates epidermal hyperplasia in ultraviolet B irradiated BALB/c mice

Tava Shelan Nagapan Ahmad Rohi Ghazali Dayang Fredalina Basri Wenna Nallance Lim   

Open Access   

Published:  Oct 31, 2018

DOI: 10.7324/JAPS.2018.81007
Abstract

Commercial sunscreens contain cosmetic preservatives which could exert phototoxic effect when exposed to sunlight. Natural sources, such as resveratrol, have gain attention in the cosmetic world as it has higher antioxidant capacity than vitamins C and E. Resveratrol also possesses strong anti-inflammatory, anti-cancer, and anti-aging properties. It also has proven to reduce epidermal hyperplasia and skin thickness. The aim of the study was to evaluate the ameliorative effect of resveratrol in reducing epidermal hyperplasia of mouse skin exposed to UVB irradiation. Eighteen female BALB/c mice were randomly divided into three groups: control group (n = 6), without Ultraviolet B irradiation and resveratrol administration; UVB exposure group (n = 6), irradiated with UVB dose of 250 mJ/cm2 for 3 minutes; and treatment group (n = 6), irradiated with UVB and treated with 0.02 ml of 200 mg/kg of resveratrol by oral gavage. Treatment was given for 14 days and UVB exposure was given on day 9, 11, and 13. On day 15, skin morphology was observed and skin-fold thickness was measured to evaluate edema. The mice were then sacrificed to obtain dorsal part of skin for histopathological observation using hematoxylin and eosin (H&E) stain. Resveratrol reduced skin scaling and erythema in UVB induced mice. Skin-fold thickness of resveratrol decreased significantly (p = 0.001) as compared to UVB irradiated group. H&E staining showed that resveratrol group reduced leukocyte infiltration and epidermal hyperplasia as compared to UVB exposure group. Hence, oral resveratrol was able to reduce skin thickness and epidermal hyperplasia and it has the potential to be developed as a natural alternative for photoprotection.


Keyword:     Ultraviolet radiation skin resveratrol BALB/c mice.


Citation:

Nagapan TS, Ghazali AR, Basri DF, Lim WN. Oral administration of resveratrol ameliorates epidermal hyperplasia in ultraviolet B irradiated BALB/c mice. J App Pharm Sci, 2018; 8(10): 047-052.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Reference

Afaq F, Adhami VM, Ahmad N. Prevention of short-term ultraviolet B radiation-mediated damages by resveratrol in SKH-1 hairless mice. Toxicol Appl Pharmacol, 2003; 186:28–37; doi:10.1016/S0041- 008X(02)00014-5

Bazzo KO, Souto AA, Lopes TG, Zanin RF, Gomez MV, Souza AH, et al. Evidence for the analgesic activity of resveratrol in acute models of nociception in mice. J Nat Prod, 2013; 76:13–21; doi:10.1021/ np300529x

D'Orazio J, Jarrett S, Amaro-Ortiz A, Scott T. UV radiation and the skin. Int J Mol Sci, 2013; 14:12222–48; doi:10.3390/ijms140612222https://doi.org/10.3390/ijms140612222

De Gruijl FR. Photocarcinogenesis: UVA vs. UVB radiation. Skin Pharmacol Appl Skin Physiol, 2002; 15:316–20; doi:10.1159/000064535https://doi.org/10.1159/000064535

El-Abaseri TB, Hansen LA. EGFR activation and ultraviolet light-induced skin carcinogenesis. J Biomed Biotechnol, 2007; 1–4; doi:10.1155/2007/97939https://doi.org/10.1155/2007/97939

El-Abaseri TB, Putta S. Ultraviolet irradiation induces keratinocyte proliferation and epidermal hyperplasia through the activation of the epidermal growth factor receptor. Carcinogenesis, 2006; 27:225–31; doi:10.1093/carcin/bgi220https://doi.org/10.1093/carcin/bgi220

González S, Gilaberte Y, Juarranz A Oral and systemic photoprotection. Princ Pract Photoprotection, 2016; 387–403; doi:10.1007/978-3-319-29382-0_22https://doi.org/10.1007/978-3-319-29382-0_22

Gregoris E, Fabris S, Bertelle M, Grassato L, Stevanato R.. Propolis as potential cosmeceutical sunscreen agent for its combined photoprotective and antioxidant properties. Int J Pharm, 2011; 405:97–101; doi:10.1016/j.ijpharm.2010.11.052https://doi.org/10.1016/j.ijpharm.2010.11.052

Handa O, Kokura S, Adachi S, Takagi T, Naito Y, Tanigawa T, et al. Methylparaben potentiates UV-induced damage of skin keratinocytes. Toxicology, 2006; 227:62–72; doi:10.1016/j.tox.2006.07.018https://doi.org/10.1016/j.tox.2006.07.018

Harber LC, Baer RL. Mechanisms of drug photosensitivity reactions. Top Catal, 1969; 14:58–67; doi:10.1016/S0041- 008X(69)80009-8

Hasiah AH, Ghazali AR, Weber JFF, Velu S, Thomas NF, Inayat Hussain SH. Cytotoxic and antioxidant effects of methoxylated stilbene analogues on HepG2 hepatoma and Chang liver cells: implications for structure activity relationship. Hum Exp Toxicol, 2011; 30:138–44; doi:10.1177/0960327110368739https://doi.org/10.1177/0960327110368739

Hsu SC, Huang SM, Chen A, Sun CY, Lin SH, Chen JS, et al. Resveratrol increases anti-aging Klotho gene expression via the activating transcription factor 3/c-Jun complex-mediated signaling pathway. Int J Biochem Cell Biol, 2014; 53:361–71; doi:10.1016/j.biocel.2014.06.002https://doi.org/10.1016/j.biocel.2014.06.002

Ichihashi M, Ueda M, Budiyanto A, Bito T, Oka M, Fukunaga M, et al. UV-induced skin damage. Toxicology, 2003; 189:21–39; doi:10.1016/ S0300-483X(03)00150-1

Jeon HY, Kim JK, Kim WG, Lee SJ. Effects of oral epigallocatechin gallate supplementation on the minimal erythema dose and uv-induced skin damage. Skin Pharmacol Physiol, 2009: 22:137–41; doi:10.1159/000201562https://doi.org/10.1159/000201562

Kajiya K, Hirakawa S, Detmar M. Vascular endothelial growth factor-A mediates ultraviolet B-induced impairment of lymphatic vessel function. Am J Pathol, 2006; 169:1496–503; doi:10.2353/ajpath.2006.060197https://doi.org/10.2353/ajpath.2006.060197

Katiyar SK, Meeran SM. Obesity increases the risk of UV radiation-induced oxidative stress and activation of MAPK and NF-kappaB signaling. Free Radic Biol Med, 2007; 42:299–310; doi:10.1016/j.freeradbiomed.2006.10.049https://doi.org/10.1016/j.freeradbiomed.2006.10.049

Khandelwal AR, Rong X, Moore-Medlin T, Ekshyyan O, Abreo F, Gu X, et al. Photopreventive effect and mechanism of AZD4547 and curcumin C3 complex on UVB-induced epidermal hyperplasia. Cancer Prev Res 2016; 9:296–304; doi:10.1158/1940-6207.CAPR-15-0366https://doi.org/10.1158/1940-6207.CAPR-15-0366

Kim HK. Garlic supplementation ameliorates UV-Induced photoaging in hairless mice by regulating antioxidative activity and MMPs expression. Molecules, 2016; 21:1–13; doi:10.3390/molecules21010070https://doi.org/10.3390/molecules21010070

Kim KH, Back JH, Zhu Y, Arbesman J, Athar M, Kopelovich L, et al. Resveratrol targets transforming growth factor- b 2 signaling to block UV-induced tumor progression. J Invest Dermatol, 2010; 131:195–202; doi:10.1038/jid.2010.250https://doi.org/10.1038/jid.2010.250

Lanzilli G, Cottarelli A, Nicotera G, Guida S, Ravagnan G, Fuggetta MP. Anti-inflammatory effect of resveratrol and polydatin by in vitro IL-17 modulation. Inflammation, 2012; 35:240–8; doi:10.1007/ s10753-011-9310-z

Lee CW, Ko HH, Chai CY, Chen WT, Lin CC, Yen FL. Effect of Artocarpus communis extract on UVB irradiation-induced oxidative stress and inflammation in hairless mice. Int J Mol Sci, 2013; 14:3860–73; doi:10.3390/ijms14023860https://doi.org/10.3390/ijms14023860

Lee TH, Seo JO, Baek SH, Kim SY. Inhibitory effects of resveratrol on melanin synthesis in ultraviolet B-induced pigmentation in guinea pig skin. Biomol Ther, 2014; 22:35–40; doi:10.4062/biomolther.2013.081https://doi.org/10.4062/biomolther.2013.081

Martinez RM, Pinho-Ribeiro FA, Steffen VS, Caviglione CV, Vignoli JA, Barbosa DS, et al. Naringenin inhibits UVB irradiation-induced inflammation and oxidative stress in the skin of hairless mice. J Nat Prod, 2015; 78:1647–55; doi:10.1021/acs.jnatprod.5b00198https://doi.org/10.1021/acs.jnatprod.5b00198

Maruki-Uchida H, Kurita I, Sugiyama K, Sai M, Maeda K, Ito T. The protective effects of piceatannol from passion fruit (Passiflora edulis) seeds in UVB-irradiated keratinocytes. Biol Pharm Bull, 2013; 36:845–9; doi:10.1248/bpb.b12-00708https://doi.org/10.1248/bpb.b12-00708

Nichols JA, Katiyar SK. Skin photoprotection by natural polyphenols: anti-inflammatory, antioxidant and DNA repair mechanisms. Arch Dermatol Res, 2010; 10:71–83; doi:10.1007/s00403-009-1001-3https://doi.org/10.1007/s00403-009-1001-3

Ouhtit A, Muller HK, Davis DW, Ullrich SE, McConkey D, Ananthaswamy HN. Temporal events in skin injury and the early adaptive responses in ultraviolet-irradiated mouse skin. Am J Pathol, 2000; 156:201–7; doi:10.1016/S0002-9440(10)64720-7https://doi.org/10.1016/S0002-9440(10)64720-7

Park JM, Cho JK, Mok JY, Jeon IH, Kim HS, Kang HJ, et al. Protective effect of astragalin and quercetin on ultraviolet (UV)-irradiated damage in HaCaT cells and BALB/c mice. J Korean Soc Appl Biol Chem, 2012; 55:443–6; doi:10.1007/s13765-012-2072-yhttps://doi.org/10.1007/s13765-012-2072-y

Polonini HC, Lima LL, Gonçalves KM, Do Carmo AMR, Da Silva AD, Raposo NRB. Photoprotective activity of resveratrol analogues. Bioorganic Med Chem, 2013; 21:964–8; doi:10.1016/j.bmc.2012.11.052https://doi.org/10.1016/j.bmc.2012.11.052

Reagan-Shaw S, Afaq F, Aziz MH, Ahmad N. Modulations of critical cell cycle regulatory events during chemoprevention of ultraviolet B-mediated responses by resveratrol in SKH-1 hairless mouse skin. Oncogene, 2004; 23:5151–60; doi:10.1038/sj.onc.1207666https://doi.org/10.1038/sj.onc.1207666

Rodford R. Safety evaluation of preservatives. Int J Cosmetic Sci, 1997; 290:281–90.https://doi.org/10.1111/j.1467-2494.1997.tb00192.x

Sambandan DR, Ratner D. Sunscreens: an overview and update. J Am Acad Dermatol, 2011; 64:748–58; doi:10.1016/j.jaad.2010.01.005https://doi.org/10.1016/j.jaad.2010.01.005

Schneider LA, Bloch W, Kopp K, Hainzl A, Rettberg P, Wlaschek M., et al. 8-Isoprostane is a dose-related biomarker for photo-oxidative ultraviolet (UV) B damage in vivo: a pilot study with personal UV dosimetry. Br J Dermatol, 2006; 154:1147–54; doi:10.1111/j.1365- 2133.2006.07192.x

Soeur J, Eilstein J, Léreaux G, Jones C, Marrot L. Skin resistance to oxidative stress induced by resveratrol: from Nrf2 activation to GSH biosynthesis. Free Radic Biol Med, 2015; 78:213–23; doi:10.1016/j. freeradbiomed.2014.10.510

Svobodová A, Psotová J, Walterová D. Natural phenolics in the prevention of UV-induced skin damage. A review. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, 2003; 147:137–45; doi:10.5507/bp.2003.019https://doi.org/10.5507/bp.2003.019

Vostálová J, ZdaÅ™ilová A, Svobodová A. Prunella vulgaris extract and rosmarinic acid prevent UVB-induced DNA damage and oxidative stress in HaCaT keratinocytes. Arch Dermatol Res, 2010; 302:171–81; doi:10.1007/s00403-009-0999-6https://doi.org/10.1007/s00403-009-0999-6

Zhu W, Chen S, Li Z, Zhao X, Li W, Sun Y, et al. Effects and mechanisms of resveratrol on the amelioration of oxidative stress and hepatic steatosis in KKAy mice. Nutr Metab, 2014; 11:1–11; doi:10.1186/1743- 7075-11-35

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