Research Article | Volume: 8, Issue: 10, October, 2018

DOE, formulation, and optimization of Repaglinide nanostructured lipid carriers

Shady A. Swidan Zeinab N. Mansour Zeinab A. Mourad Nahla A. Elhesaisy Nada A. Mohamed Mohamed S. Bekheet Mohamed A. Badawy Mai M. Elsemeiri Aya E. Elrefaey Amera M. Hassaneen   

Open Access   

Published:  Oct 31, 2018

DOI: 10.7324/JAPS.2018.81002

Nanostructured lipid carriers (NLC) are a recent approach for the delivery of poorly soluble drugs with low oral bioavailability. The oral antidiabetic Repaglinide (RPG) was loaded into NLC using emulsification–ultrasonification technique. A design of experiment was constructed to study the formulation variables. The influence of the liquid lipid to the solid lipid ratio and the concentration of the surfactant on mean particle size, zeta potential, and drug entrapment efficiency was demonstrated. The mean particle size ranged from 182 ± 7.9 nm to 452 ± 66.1 nm. All particles were negatively charged and the zeta potential values ranged from −7.9 ± 0.9 mV to −44.4 ± 6.2 mV. The highest entrapment efficiency was obtained with the minimum solid lipid to liquid lipid ratio and lowest surfactant concentration. All RPG–NLC formulae showed biphasic time-dependent in vitro release and the studied factors were optimized and the optimum formula was evaluated for in vitro release and crystallinity. The in vitro release of the optimized formula fitted to the Higuchi diffusion model. In conclusion, this study showed the potential of NLC as a carrier for controlled release of RPG.

Keyword:     Nanostructured lipid carriers emulsification– ultrasonification Repaglinide optimization DSC.


Swidan SA, Mansour ZN, Mourad ZA, Elhesaisy NA, Mohamed NA, Bekheet MS, et al. DOE, formulation, and optimization of Repaglinide nanostructured lipid carriers. J App Pharm Sci, 2018; 8(10): 008-016.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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