Research Article | Volume: 8, Issue: 9, September, 2018

Biological potential of Citharexylum myrianthum Cham. leaves in vitro and phenolic profile by HPLC-ESI-MS/MS

Adrielli Tenfen Camile Cecconi Cechinel-Zanchett Ana Paula Dalmagro Priscila Zimath Ariela Maína Boeder Gabriel M. D. Santos Adriana Campos Diogo Alexandre Sibert Gustavo Micke Luciano Vitali Caio Maurício Mendes de Córdova Alexandre Bella-Cruz Rivaldo Niero Valdir Cechinel-Filho   

Open Access   

Published:  Sep 30, 2018

DOI: 10.7324/JAPS.2018.8911

This study aimed to evaluate the antimicrobial, cytotoxic effects in vitro and phenolic profile of Citharexylum myrianthum Cham. leaves. Dried leaves were macerated with methanol and subjected to liquid-liquid partition with solvents of increasing polarity, furnishing the methanolic extract (ME), dichloromethane (DCMF) and ethyl acetate (EAF) fractions. They were subsequently analyzed by HPLC-ESI-MS/MS. Six strains of Mycoplasma, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, and Candida albicans, were used to determine the antimicrobial effects, and minimum inhibitory concentrations (MIC) values of less than 1000 μg.mL−1 were considered active. To evaluate the cytotoxic effects, the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay (MTT) test was conducted with colorectal adenocarcinoma (HT-29), non-small lung cancer (NCI-H460) and non-cancer fibroblast (MRC5) cell lines. HPLC-ESI-MS/MS analysis identified four main phenolic compounds, vanillic, p-coumaric and salicylic acids and hispidulin. All samples were considered active against Mycoplasmas, mainly against M. hominis, with MIC values of 250 μg.mL−1. With respect to cytotoxicity, the ME and DCMF (100 μg.mL−1) reduced cell viability by 50% in both the HT-29 and NCI-H460 cell lines but were non-cytotoxic against the MRC5. These results in vitro showed that C. myrianthum Cham. may be a possible candidate as an antimicrobial and antitumor agent. However, further studies in vivo are needed to confirm its effects.

Keyword:     Antibacterialcytotoxicitymedicinal plantphytotherapyphenolic content.


Tenfen A, Cechinel-Zanchett CC, Dalmagro AP, Zimath P, Boeder AM, Santos GMD, Campos A, Sibert DA, Micke G, Vitali L, de Córdova CMM, Bella-Cruz A, Niero R, CechinelFilho V. Biological potential of Citharexylum myrianthum Cham. leaves in vitro and phenolic profile by HPLC-ESI-MS/ MS. J App Pharm Sci, 2018; 8(09): 074-080.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Abu-Darwish MS, Efferth T. Medicinal plants from near east for cancer therapy. Front Pharmacol, 2018; 31:9-56.

Almeida IV, Cavalcante FM, Vicentini VE. Different responses of vanillic acid, a phenolic compound, in HTC cells: cytotoxicity, antiproliferative activity, and protection from DNA-induced damage. Genet Mol Res, 2016; 15(4).

Amaral WAN, Antiqueira LMOR, Horbach MA. Frutification and germination ecology of Citharexylum myrianthum Cham (Verbenaceae). J Biotec Biodivers, 2013; 4:207-215.

Antunes RMP, Lima EO, Pereira MSV, Camara CA, Arrudal TA, Catão RMR, Barbosa TP, Nunes XP, Dias CS, Silva TMS. Atividade antimicrobiana "in vitro" e determinação da concentração inibitória mínina (CIM) de fitoconstituintes e produtos sintéticos sobre bactérias e fungos leveduriformes. Rev Bras Farmacog, 2006; 16:517-524.

Ayers A, Sneden AT. Caudatosides A-F: new iridoid glucosides from Citharexylum caudatum. J Nat Prod, 2002; 65:1621-1626.

Balázs B, Tóth G, Duddeck H, Soliman HS. Iridoid and lignan glycosides from Citharexylum spinosum L. Nat Prod Res, 2006; 20:201- 205.

Barizão EO, Visentainer JV, Almeida VC, Ribeiro D, Chisté RC, Fernandes E. Citharexylum solanaceum fruit extracts: Profiles of phenolic compounds and carotenoids and their relation with ROS and RNS scavenging capacities. Food Res Int, 2016; 86:24-33.

Bébéar C, Robertson J. 1996. Molecular and diagnostic procedures in mycoplasmology. 2 ed. California, U.S.A.: Academic Press.

Bosse TS. Fitoterápicos no SUS. 2014. (Monography). Pós-graduação em Farmacologia, Universidade do Extremo Sul Catarinense – UNESC.

CLSI. Methods for Dilution Antimicrobial Susceptibility Tests f or Bacteria That Grow Aerobically; Approved Standard – Ninth Edittion. CLSI document M97-A9. Wayne, PA: Clinical and Laboratory Standards Institute; 2012.

Deguchi T, Ito S, Yasuda M, Kondo H, Yamada Y, Nakane K, Mizutani K, Tsuchiya T, Yokoi S, Nakano M. Emergence of Mycoplasma genitalium with clinically significant fluoroquinolone resistance conferred by amino acid changes both in GyrA and ParC in Japan. J Infect Chemother, 2017; 23:648-650.

Eller SCWS, Feitosa VA, Arruda TA, Antunes RMP, Catão RMR. Avaliação antimicrobiana de extratos vegetais e possível interação farmacológica in vitro. Rev Ciênc Farm Bas Apl, 2015; 36:131-136.

Ganapaty S, Rao DV, Pannakal ST. A phenethyl bromo ester from Citharexylum fruticosum. Nat Prod Commun, 2010; 5:399-402.

Gao H, Liu Y, Li K, Wu T, Peng J, Jingó F. Hispidulin induces mitochondrial apoptosis in acute myeloid leukemia cells by targeting extracellular matrix metalloproteinase inducer. Am J Transl Res, 2016; 8:1115-1132.

Gobbo-Neto L, Lopes NP. Plantas medicinais: fatores de influência no conteúdo de metabólitos secundários. Quim Nova, 2007; 30:374-381.

Hamed ANE, Muhammad MHH, Khalil HE, Kamel MS. 2014. Biological studies of Citharexylum quadrangulare jacq. family Verbenaceae. Assiut Univ. 9th International Pharmaceutical Sciences Conference 2014. [ONLINE] Available at: publication/274376180_Assiut_Citharexylum_Bio>Abstract_form.

Holetz FB, Penssini GL, Sanches NR, Cortez DAG, Nakamura C, Dias-Filho BP. Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases. Mem Inst Oswaldo Cruz, 2002; 97:1027-1031.

Intisar A, Zhang L, Luo H, Kiazolu JB, Zhang R, Zhang W. Anticancer constituents and cytotoxic activity of methanol-water extract of Polygonum bistorta L. Afr J Tradit Complement Altern Med, 2012; 10:53- 59.

Ipe – Instituto de Pesquisas Ecológicas. Citharexylum myrianthum Cham. 2016. [ONLINE] Available at:

Johnstone RW, Ruefli AA, Lowe SW. Apoptosis: a link between cancer genetics and chemotherapy. Cell, 2002; 108:153-64.

Khan MR, Siddique F. Antioxidant effects of Citharexylum spinosum in CClâ‚„ induced nephrotoxicity in rat. Exp Toxicol Pathol, 2012; 64:349-355.

Mar A, Pripdeevech P. Chemical composition and antibacterial activity of essential oil and extracts of Citharexylum spinosum flowers from Thailand. Nat Prod Commun, 2014; 9:707-10.

Michelin DC, Moreschi PE, Lima AC, Nascimento GGF, Paganelli MO, Chaud MV. Avaliação da atividade antimicrobiana de extratos vegetais. Rev Bras Farmacogn, 2005; 15:316-320.

Mohammed MHH, Hamed ANES, Khalil HE, Kamel MS. Phytochemical and pharmacological studies of Citharexylum quadrangulare Jacq. Leaves. J Med Plants Res, 2016; 10(18).

Muraiana IA, Picard J, Eloff JN. Development of a reproducible method to determine minimum inhibitory concentration (MIC) of plant extract against a slow-growing mycoplasmas organism. Phytomed, 2009; 16:262-264.

Murray PR, Baron EJ. 2007. Manual of Clinical Microbiology. 9 ed. Washington, U.S.A.: ASM Press.

Newman DJ, Cragg GM. Natural products as sources of new drugs from 1981 to 2014. J Nat Prod, 2016; 79:629-661.

Rasheeda K, Bharathy H, Nishad Fathima N. Vanillic acid and syringic acid: Exceptionally robust aromatic moieties for inhibiting in vitro self-assembly of type I collagen. Int J Biol Macromol, 2018; 113:952-960.

Rocca-de-Andrade MA. Biologia da polinização da espécie arbórea Citharexylum myrianthum Cham. (Verbenaceae), polinizadores e utilização do recurso floral pelos visitantes. 2001. Thesis (Master's Degree) – Instituto de Biologia, Programa de Pós-Graduação em Ecologia, Universidade Estadual de Campinas – UNICAMP, Campinas.

Rosa LS, Monteiro MC, Silva NJ, Teodoro AJ. Antioxidant activity of phenolic acids and cytotoxic effect in human adenocarcinoma cells. 11 SLACA, 2015; 2.

Saidi I, Waffo-Téguo P, Ayeb-Zakhama AEL, Harzallah-Skhiri F, Marchal A, Ben Jannet H. Phytochemical study of the trunk bark of Citharexylum spinosum L. growing in Tunisia: Isolation and structure elucidation of iridoid glycosides. Phytochem, 2018; 146:47-55.

Seoane S, Montero JC, Oca-a A, Pandiella A. Effect of multikinase inhibitors on caspase-independent cell death and DNA damage in HER2-overxpressing breast cancer cells. J Natl Cancer Inst, 2010; 102:1432-1446.

Shulz M, Borges GSC, Gonzaga LV, Seraglio SKT, Olivo IS, Azevedo MS, Nehring P, Gois JS, Almeida TS, Vitali L, Spudeit DA, Micke GA, Borges DLG, Fett R. Chemical composition, bioactive compounds and antioxidant capacity of juçara fruit (Euterpe edulis Martius) during ripening. Food Res International, 2015; 77:125-131.

Velleca W, Bird B, Forrester F. 1979. Laboratory diagnosis of Mycoplasma infections. Course 8226-C, U.S. Department of Health, Education and Welfare, Public Health Service, Centers for Disease Control, Atlanta, U.S.A.

Wang YG, Liu WP, He XS, Fei Z. Hispidulin enhances the anti-tumor effects of temozolomide in glioblastoma by activating AMPK. Cell Biochem Biophys, 2015; 71:701-706.

Yemiþ GP, Pagotto F, Bach S, Delaquis P. Effect of vanillin, ethyl vanillin, and vanillic acid on the growth and heat resistance of Cronobacter species. J Food Prot, 2011; 74:2062-2069.

Yu J, Liu H, Lei J, Tan W, Hu X, Zou G. Antitumor activity of chloroform fraction of Scutellaria barbata and its active constituents. Phytother Res, 2007; 21:817-22.

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