Research Article | Volume: 8, Issue: 8, August, 2018

Understanding the role of alkaline phosphatase as a possible marker for the evaluation of antiparasitic agents

Gustavo A. Martínez Luis E. Trujillo   

Open Access   

Published:  Aug 31, 2018

DOI: 10.7324/JAPS.2018.8817

The release of alkaline phosphatase (AP) into media provides an enzymatic method for the evaluation of the effectiveness of antiparasitic drugs in vitro. However, to date, it has only been applied sporadically in some cestodes and trematodes. In this paper, we describe for the first time a cost-effective evaluation method to accurately determine larvae immobility or mortality after salt or starvation stress treatment in order to measure the released parasitic AP as an enzymatic stress marker. Our method avoids tedious and time-consuming visual counting of the model organism, human parasite nematode Strongyloides venezuelensis larvae. In opposition to previous reports, we found a linear relationship between larval immobilization due to stress treatment and the released AP from L3 larvae. This method could be applied to a preclinical evaluation of active antiparasitic compounds in AP producing nematodes and other parasites.

Keyword:     Alkaline phosphatase larval mortality Strongyloides venezuelensis parasites larvae immobility.


Martínez GA, Trujillo LE. Understanding the role of alkaline phosphatase as a possible marker for the evaluation of antiparasitic agents. J App Pharm Sci, 2018; 8(08): 123-128.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Adnani-Sadati SJ, Farahnak A, Molaei-Rad MB, Golestani A, Eshraghiyan MR. A Comparison between the Effects of Albendazole and Mebendazole on the Enzymatic Activity of Excretory / Secretory Products of Echinococcus granulosus Protoscoleces in Vitro. Iranian J Public Health, 2016; 45(2):223-229.

Anya AO. The structure and chemical composition of the nematode cuticle. Observations on some oxyurids and Ascaris. Parasitology, 1966; 56(1):179-198.

Bird A, Bird J. 1991. The Structure of Nematodes. California, United States of America: Academic Press, Inc.

Bird AF, Deutsch K. The structure of the cuticle of Ascaris lumbricoides var. suis. Parasitology, 1957; 47(3–4):319-328.

Coleman JE. Structure and mechanism of alkaline phosphatase. Annu Rev Biophys Biomol Struct, 1992; 21:441-483.

Grove DI, Warton A, Northern C, Papadimitriou JM. Electron Microscopical Studies of Strongyloides ratti Infective Larvae: Loss of the Surface Coat during Skin Penetration. J Parasitol, 1987; 73(5):1030-1034.

Horiuchi T, Horiuchi S, Mizuno D. A possible negative feedback phenomenon controlling formation of alkaline phosphomonoesterase in Escherichia coli. Nature, 1959; 183:1529-1530.

Lee DL. The cuticle of adult Nippostrongylus brasiliensis. Parasitology, 1965; 55(1):173-181.

Mahanty S, Paredes A, Marzal M, Gonzalez E, Rodriguez S, Dorny P, Guerra-Giraldez C, Garcia HH, Nash T. Sensitive in vitro system to assess morphological and biochemical effects of praziquantel and albendazole on Taenia solium cysts. Antimicrob Agents Chemother, 2011; 55(1):211-217.

Martinez AM, De-Souza W. A quick-frozen, freeze-fracture and deep-etched study of the cuticle of adult forms of Strongyloides venezuelensis (Nematoda). Parasitology, 1995; 111:523-529.

Page AP, Stepek G, Winter AD, Pertab D. Enzymology of the nematode cuticle: A potential drug target? Int J Parasitol Drugs and Drug Resist, 2014; 4(2):133-141.

Paredes A, de Campos Lourenço T, Marzal M, Rivera A, Dorny P, Mahanty S, Guerra-Giraldez C, García HH, Nash TE, Cass QB. In vitro analysis of albendazole sulfoxide enantiomers shows that (+)-(R)- albendazole sulfoxide is the active enantiomer against Taenia solium. Antimicrob Agents Chemother, 2013; 57(2):944-949.

Ruano A. 2008. Óxido nítrico como modulador de la estrongiloidosis. Universidad de Salamanca. Available at: http://hdl.handle. net/10366/17706. [Accessed 28 March 2018].

Sayers G, Mackenzie CD, Denham DA. Biochemical surface components of Brugia pahangi microfilariae. Parasitology, 1984; 89(3):425- 434.

Sood ML, Kalra S. Histochemical studies on the body wall of nematodes: Haemonchus contortus (Rud., 1803) and Xiphinema insigne Loos. Z Parasitenkd, 1977; 51(3):265-273.

Stettler M, Siles-Lucas M, Sarciron E, Lawton P, Gottstein B, Hemphill A. Echinococcus multilocularis Alkaline Phosphatase as a Marker for Metacestode Damage Induced by In Vitro Drug Treatment with Albendazole Sulfoxide and Albendazole Sulfone. Antimicrobial Agents and Chemotherapy. 2001; 45(8):2256-2262.

Swargiary A, Roy B, Ronghang B. Partial characterization of alkaline phosphatase in Fasciolopsis buski – an intestinal fluke treated with crude extract of Alpinia nigra (Zingiberaceae). Journal of Pharmaceutical Technology and Drug Research, 2013; 2:5.

Watson BD. The fine structure of the body-wall in a free-living nematode, Euchromadora vulgaris. Journal of Cell Science, 1965; s3- 106:75-81.

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