Research Article | Volume: 8, Issue: 8, August, 2018

Comparative study of Mentha species growing wild in Egypt: LC-ESI-MS analysis and chemosystematic significance

Mona M. Marzouk Sameh R. Hussein Ahmed Elkhateeb Mona El-shabrawy El-Sayed S. Abdel-Hameed Salwa A. Kawashty   

Open Access   

Published:  Aug 31, 2018

DOI: 10.7324/JAPS.2018.8816

Mentha longifolia (L.) Huds. and Mentha pulegium L. are growing wild in Egyptian flora, they have many biological activities and commonly used as traditional herbal medicines. The aqueous methanolic extracts of the two Egyptian species were subjected to a comparative study for the first time using the LC-ESI-MS fingerprint. 43 compounds belonging to different chemical classes (flavone, flavonol, flavanone, C-glycosylflavone, phenolic acids and their glycosides) have been identified. The identified compounds can be used as chemosystematic markers and further our knowledge of the infraspecific relationship between the studied species. Further isolation and in vitro testing of metabolites will provide more evidence for the possible uses of Mentha sp.

Keyword:     Mentha longifolia Mentha pulegium LC-ESI-MS Flavonoids Chemosystematics.


Marzouk MM, Hussein SR, Elkhateeb A, El-shabrawy M, Abdel-Hameed ES, Kawashty SA. Comparative study of Mentha species growing wild in Egypt: LC-ESI-MS analysis and chemosystematic significance. J App Pharm Sci, 2018; 8(08): 116-122.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text


Akroum S, Bendjeddou D, Satta D, Lalaoui K. Antibacterial activity and acute toxicity effect of flavonoids extracted from Mentha longifolia. AEJSR, 2009; 4:93-96.

Al-Ali KH, El-Beshbishy HA, El-Badry AA, Alkhalaf M. Cytotoxic activity of methanolic extract of Mentha longifolia and Ocimum basilicum against human breast cancer. PJBS, 2013; 16:1744-50.

Ali MS, Saleem M, Ahmad W, Parvez M, Yamdagni R. A chlorinated monoterpene ketone, acylated β-sitosterol glycosides and a flavanone glycoside from Mentha longifolia (Lamiaceae). Phytochemistry, 2002; 59:889-95.

Areias FM, Valentao P, Andrade PB, Ferreres F, Seabra RM. Phenolic fingerprint of peppermint leaves. Food Chemistry, 2001; 73(3):307-311.

Baris O, Karadayi M, Yanmis D, Guvenalp Z, Bal T, Gulluce M. Isolation of 3 flavonoids from Mentha longifolia (L.) Hudson subsp. longifolia and determination of their genotoxic potentials by using the E. coli WP2 test system. J. Food Sci, 2011; 76:9.

Brahmi F, Khodir M, Mohamed C, Pierre D. Chemical Composition and Biological Activities of Mentha Species. In Aromatic and Medicinal Plants-Back to Nature. InTech, 2017; 47-80.

Aksit H, Çelik SM, Sen Ö, Erenler R, Demirtas I, Telci I, Elmastas M. Complete isolation and characterization of polar portion of Mentha dumetorum water extract. Rec Nat Prod, 2014; 8:277.

Areias FM, Valentao P, Andrade PB, Ferreres F, Seabra RM. Phenolic fingerprint of peppermint leaves. Food Chem, 2001; 73:307-311.

Berdowska I, Zieliński B, Fecka I, Kulbacka J, Saczko J, Gamian A. Cytotoxic impact of phenolics from Lamiaceae species on human breast cancer cells. Food Chem, 2013; 141:1313-1321.

Chen HJ, Inbaraj BS, Chen BH. Determination of phenolic acids and flavonoids in Taraxacum formosanum Kitam by liquid chromatography-tandem mass spectrometry coupled with a post-column derivatization technique. Int J Mol Sci, 2011; 13:260-285.

El-Ghorab AH. The chemical composition of the Mentha pulegium L. essential oil from Egypt and its antioxidant activity. J Essent Oil Bear Pl, 2006; 9:183-95.

Farag MA, Otify A, Porzel A, Michel CG, Elsayed A, Wessjohann LA. Comparative metabolite profiling and fingerprinting of genus Passiflora leaves using a multiplex approach of UPLC-MS and NMR analyzed by chemometric tools. Anal Bioanal Chem, 2016; 408:3125-3143.

Gulluce M, Orhan F, Adiguzel A, Bal T, Guvenalp Z, Dermirezer LO. Determination of antimutagenic properties of apigenin-7-O-rutinoside, a flavonoid isolated from Mentha longifolia (L.) Huds. ssp. longifolia with yeast DEL assay. Toxicol Ind Health, 2013; 29:534-40.

Gulluce M, Orhan F, Yanmis D, Arasoglu T, Guvenalp Z, Demirezer LO. Isolation of a flavonoid, apigenin 7-O-glucoside, from Mentha longifolia (L.) Hudson subspecies longifolia and its genotoxic potency. Toxicol Ind Health. 2015; 31:831-40.

Guvenalp Z, Ozbek H, Karadayi M, Gulluce M, Kuruuzum-Uz A, Salih B, Demirezer O. Two antigenotoxic chalcone glycosides from Mentha longifolia subsp. longifolia. Pharm Biol, 2015; 53:888-96.

Hajlaoui H, Trabelsi N, Noumi E, Snoussi M, Fallah H, Ksouri R, Bakhrouf A. Biological activities of the essential oils and methanol extract of tow cultivated mint species (Mentha longifolia and Mentha pulegium) used in the Tunisian folkloric medicine. World J Microbiol Biotechnol, 2009; 25:2227-2238.

Harley RM, Brighton CA. Chromosome numbers in the genus Mentha L. Bot J Linn Soc, 1977; 74:71-96.

Hussein SR, Abdel Latif RR, Marzouk MM, Elkhateeb A, Mohammed RS, Soliman AAF, Abdel-Hameed ES. Spectrometric analysis, phenolics isolation and cytotoxic activity of Stipagrostis plumosa (Family Poaceae). Chem Pap, 2018; 72:29-37.

Mint JF. In, Biotechnology in Agriculture and Forestry, Transgenic Crops IV. Pua EC, Davey MR (Eds.), Springer-Verlag Berlin Heidelberg, 2007. Vol. 59.

Kapp K, Hakala E, Orav A, Pohjala L, Vuorela P, Püssa T, Vuorela H, Raal A. Commercial peppermint (Mentha× piperita L.) teas: Antichlamydial effect and polyphenolic composition. Food Res Int, 2013; 53:758-66.

Kapp K. Polyphenolic and essential oil composition of Mentha and their antimicrobial effect. Faculty of Pharmacy of the University of Helsinki, 2015; 1-73.

Krzyzanowska J, Janda B, Pecio L, Stochmal A, Oleszek W, Czubacka A, Przybys M, Doroszewska T. Determination of polyphenols in Mentha longifolia and M. piperita field-grown and in vitro plant samples using UPLC-TQ-MS. Journal of AOAC International, 2011; 94:43-50.

Lawrence BM. Mint: the genus Mentha. Medicinal and aromatic plants-industrial profiles. CRC Press/Taylor & Francis, Boca Raton, FL. 2007.

Marzouk MM, Hussein SR, Elkhateeb A, Farid MM, Ibrahim LF, Abdel-Hameed ES. Phenolic profiling of Rorippa palustris (L.) Besser (Brassicaceae) by LC-ESI-MS: Chemosystematic significance and cytotoxic activity. Asian Pac J Trop Dis, 2016; 6:633-637.

Mustafa AZ, Badr A, El-Galaly MA, Mobarak AA, Hassan MG. Genetic diversity among Mentha populations in Egypt as reflected by isozyme polymorphism. Int J Bot, 2005; 1:188-195.

Mustafa AM, Badr A, El-Galaly MA, Mobarak AA, Hassan MG. Genetic diversity among Ocimum populations in Egypt as reflected by morphological, seed proteins and isozyme polymorphism. Int J Bot, 2006; 2:261.

Naghibi F, Mosaddegh M, Mohammadi Motamed M, Ghorbani A. Labiatae family in folk medicine in Iran: from ethnobotany to pharmacology. Iran J Pharm Res, 2010; 4:63-79.

Ncube EN, Mhlongo MI, Piater LA, Steenkamp PA, Dubery IA, Madala NE. Analyses of chlorogenic acids and related cinnamic acid derivatives from Nicotiana tabacum tissues with the aid of UPLC-QTOF-MS/MS based on the in-source collision-induced dissociation method. Chem Cent J, 2014; 8:66.

Orhan F, Barış Ö, Yanmış D, Bal T, Güvenalp Z, Güllüce M. Isolation of some luteolin derivatives from Mentha longifolia (L.) Hudson subsp. longifolia and determination of their genotoxic potencies. Food Chem, 2012; 135:764-769.

Püssa T, Raudsepp P, Toomik P, Pällin R, Mäeorg U, Kuusik S, Soidla R, Rei M. A study of oxidation products of free polyunsaturated fatty acids in mechanically deboned meat. J. Food Comp. Anal, 2009; 22:307- 314.

Shalaby NMM, Moharram FA, El-Toumy SAA, Marzouk MSA, Ahmed AAE. Phytochemical and pharmacological studies of Mentha pulegium L. Bulletin of Faculty of Pharmacy, Cairo University, Department of Natural Products, National Research Centre, Cairo, Egypt, 2000; 38: 143–151.

Sharaf M, El-Ansari MA, Saleh NA. Flavone glycosides from Mentha longifolia. Fitoterapia, 1999; 70:478-483.

Shirazi FH, Ahmadi N, Kamalinejad M. Evaluation of northern Iran Mentha pulegium L. cytotoxicity. DARU J Pharm. Sci, 2004; 12:106- 110.

Stocker M, Pohl R. Postmortale bildung von 5, 7-dihydroxychromon-7-rutinosid in Mentha longifolia. Phytochemistry, 1976; 15:571-572.

Taamalli A, Arráez-Román D, Abaza L, Iswaldi I, Fernández- Gutiérrez A, Zarrouk M, Segura-Carretero A. LC-MS-based metabolite profiling of methanolic extracts from the medicinal and aromatic species Mentha pulegium and Origanum majorana. Phytochem Anal, 2015; 26:320-330.

Tomás-Barberán FA, Husain SZ, Gil MI. The distribution of methylated flavones in the Lamiaceae. Biochem Syst Ecol, 1988; 16:43- 46.

Voirin B, Bayet C, Faure O, Jullien F. Free flavonoid aglycones as markers of parentage in Mentha aquatica, M. citrata, M. spicata and M. x piperita. Phytochemistry, 1999; 50:1189-1193.

Xu LL, Xu JJ, Zhong KR, Shang ZP, Wang F, Wang RF, Zhang L, Zhang JY, Liu B. Analysis of non-volatile chemical constituents of menthae haplocalycis herba by ultra-high performance liquid chromatography-high resolution mass spectrometry. Molecules, 2017; 22:1756.

Zaidi F, Voirin B, Jay M, Viricel MR. Free flavonoid aglycones from leaves of Mentha pulegium and Mentha suaveolens (Labiatae). Phytochemistry, 1998; 48:991-994.

Zhao GR, Zhang HM, Ye TX, Xiang ZJ, Yuan YJ, Guo ZX, Zhao LB. Characterization of the radical scavenging and antioxidant activities of danshensu and salvianolic acid B. Food Chem Toxicol, 2008; 46:73-81.

Article Metrics
555 Views 112 Downloads 667 Total



Related Search

By author names