Research Article | Volume: 8, Issue: 8, August, 2018

Coumarins from Creston Apple Seeds: Isolation, Chemical Modification, and Cytotoxicity Study

Yasser Fakri Mustafa Moath Abdulla Najem Zena Sideek Tawffiq   

Open Access   

Published:  Aug 31, 2018

DOI: 10.7324/JAPS.2018.8808
Abstract

In the present work, preliminary phytochemical screening tests were performed on extracts of Creston apple seeds. Extraction was performed using two methods, which are serial Soxhlet extraction and kinetic maceration utilizing water, methanol, chloroform, and n-hexane as extraction solvents. Three coumarin derivatives acquired from chloroform extract in the order of increasing polarity were isolated via column chromatography and one of them was chemically modified by esterification, Fries rearrangement, and methylation afforded three semisynthetic derivatives. Detection of physicochemical properties and analysis of FTIR, 1H-NMR and 13C-NMR spectra of the natural and semisynthetic coumarins were used to identify their structures. In vitro cytotoxic activity of the aforementioned coumarin derivatives was studied on three cancer cell lines, which are MCF-7, AMN3, and HeLa using MTT assay. The results indicated that compounds N3, S4, S5 and S6 have IC50 values lower than that of 5- fluorouracil against MCF-7 cell line and all compounds have higher IC50 values than that of 5-fluorouracil against AMN3 and HeLa cell lines.


Keyword:     Creston applephytochemical coumarinderivative cytotoxicity.


Citation:

Mustafa YF, Najem MA, Tawffiq ZS. Coumarins from Creston Apple Seeds: Isolation, Chemical Modification, and Cytotoxicity Study. J App Pharm Sci, 2018; 8(08): 049-056.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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