Research Article | Volume: 8, Issue: 7, July, 2018

LC-ESI-MS analysis, antitumor and antioxidant activities of methanolic extract of Egyptian Allium kurrat

Heba Abdel-Hady Mortada Mohamed El-Sayed Mahfouz Mohamed Abdel-Gawad Eman Ahmed El-Wakil El-Sayed Saleh Abdel-Hameed Ezzat El-Sayed Abdel-Lateef   

Open Access   

Published:  Jul 30, 2018

DOI: 10.7324/JAPS.2018.8714

Human have been constantly using plants to overcome many diseases. Allium kurrat is an edible cultivated vegetable in Egypt. The aim of this survey is to determine total phenolic and flavonoid contents of the methanol extract and certainly obtained fractions from it. Antioxidant activity of these extracts was evaluated by DPPH (2,2-diphenyl-1- picrylhydrazyl radical), ABTS (2,2-azino-bis-3-ethylbenzothialozine-sulphonic acid) and total antioxidant capacity assays. Also, cytotoxic activity of these extracts was determined against human hepatocellular carcinoma (HepG2) and human colon carcinoma (Caco-2) using Neutral red assay. LC-ESI-MS analysis was carried out for the most active extracts. The results revealed that ethyl acetate fraction has the highest phenolic and flavonoid contents (127.45 ± 0.71 (mg GAE eq./g extract) and 98.55 ± 1.87 (mg RE eq./g extract)) as well as showed the highest antioxidant activity. On the other hand, the butanolic fraction had the highest antitumor activity on HepG2 and Caco-2 cell lines (36.2 μg/ mL and 44.6 μg/mL) respectively. These results proved that the antitumor activity of this plant is correlated with many active ingredients in it. The LC-ESI-MS analysis identified a mixture of phenolic compounds (flavonoid glycosides and phenolic acids). Thus, Allium kurrat contains active ingredients which inhibit the risk of many chronic diseases.

Keyword:     Allium kurrat antioxidant HePG2 Coca-2 LC-ESI-MS phenolic compounds.


Abdel-Hady H, El-Sayed MM, Abdel-Gawad MM, El-Wakil EA, Abdel-Hameed ES, Abdel-Lateef EEL. LC-ESI-MS analysis, antitumor and antioxidant activities of methanolic extract of Egyptian Allium kurrat. J App Pharm Sci, 2018; 8(07): 085-092.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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