Research Article | Volume: 8, Issue: 4, April, 2018
Research Article | Volume: 8, Issue: 4, April, 2018
Green tea (Camellia sinensis L.) contains bioactive compounds such as epigallocatechin gallate (EGCG), caffeine, and gallic acid. The study aimed to optimize the extraction condition using the experimental design of factorial design. Two variables namely water temperature (75 and 95oC) and brewing number (one and two-times) were used and objected to factorial design in order to get the optimum condition. The determination of EGCC, caffeine, and gallic acid was carried out using high-performance liquid chromatography method equipped with the UV-visible detector. The result showed that the extraction yield varied from 4.48%-7.56%. The level of EGCG and caffeine in green tea extract varied from 251.96-393.34 mg/g dry weight and 32.94-46.82 mg/g dry weight, while the level of gallic acid could not be quantified in each experiment because it was below the limit of quantification (LOQ). The predicted optimum extraction condition consisted of water temperature at 95oC with two-times brewing. Using this optimum condition, the concentrations of EGCG, caffeine and the extraction yield were of 356.43 mg/g dry weight, 38.76 mg/g dry weight, and 5.76%, respectively.
Setyawan EI, Rohman A, Setyowati EP, Nugroho AK. Application of Factorial Design on the Extraction of Green Tea Leaves (Camellia sinensis L.). J App Pharm Sci, 2018; 8(04): 131-138.
Year
Month
Formulation and optimization of solid dispersion of Clopidogrel with PEG 6000
Shailendra Kumar Singh, Soukarya Som, Upender ShankhwarProcess optimization of efavirenz film coated tablet
K. Bala Krishna, M. SarithaGreen Tea Attenuates Experimental Hepatitis in Context of Oxidative Stress
Cytotoxic activity of methanolic extract of Artocarpus heterophyllus against A549, Hela and MCF-7 cell lines
Rajesh M.Patel, Sahil K. Patel