Research Article | Volume: 8, Issue: 4, April, 2018

Application of capillary electrophoresis coupling with electrochemiluminescence detection to estimate activity of butyrylcholinesterase

Dongdong Wang Fanlin Li Ming Su Hanwen Sun   

Open Access   

Published:  Apr 29, 2018

DOI: 10.7324/JAPS.2018.8402
Abstract

A new method to estimate the butyrylcholinesterase (BChE, EC 3.1.1.8) activity by using capillary electrophoresis (CE) coupled with electrochemiluminescence (ECL) is described. The liberated N,N-dimethylethanolamine (DMEA) that is produced by BChE catalyzed hydrolysis reaction of tetracine hydrochloride was used as ECL coreagent to enhance Ru(bpy)32+ ECL signals efficiently. The detection limit for DMEA was 1.98 × 10−8 M (S/N = 3), which is equal to 6.6 × 10−10 units of BChE used to catalyze substrate for 30 mins. The Michaelis constant Km (1.16 × 10−3 mol/L) and the maximum reaction velocity Vmax (2.71 × 10−7 mol/L/min) of BChE for tetracine hydrochloride are determined. Also, the reaction conditions including the concentration of metal ions, incubation temperature and pH were optimized. This method was successfully applied to detect BChE activity as a bio-marker in plasma and the results were in good agreement with that obtained by the clinical method.


Keyword:     Capillary electrophoresis electrochemiluminescence butyrylcholinesterase enzyme activity.


Citation:

Wang D, Li F, Su M, Sun H. Application of capillary electrophoresis coupling with electrochemiluminescence detection to estimate activity of butyrylcholinesterase. J App Pharm Sci, 2018; 8(04): 007-014.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text

Reference

Article Metrics
389 Views 39 Downloads 428 Total

Year

Month

Related Search

By author names