Research Article | Volume: 8, Issue: 3, March, 2018

Chemical study and evaluation of antioxidant activity and α-glucosidase inhibition of Myrciaria strigipes O. Berg (Myrtaceae)

Rafael D. Faitanin,  João V. D. Gomes,  Patrícia M. Rodrigues,  Luís Fernando T. de Menezes,  Álvaro C. Neto,  Rita C. R. Gonçalves,  Rodrigo R. Kitagawa,  Dâmaris Silveira,  Claudia M. Jamal   
Rafael D. Faitanin1, João V. D. Gomes2, Patrícia M. Rodrigues3, Luís Fernando T. de Menezes3, Álvaro C. Neto4, Rita C. R. Gonçalves1, Rodrigo R. Kitagawa1, Dâmaris Silveira2, Claudia M. Jamal1

1Department of Pharmaceutical Sciences, Federal University of Espírito Santo, Campus Maruípe, Vitória, Brazil.

2Department of Pharmacy, Faculty of Health Sciences, University of Brasilia, Campus Darcy Ribeiro, Brasília, Brazil.

3Department of Agrarian and Biological Sciences, Federal University of Espírito Santo, Campus São Mateus, São Mateus, Brazil.

4Department of Chemistry, Federal University of Espírito Santo, Campus Goiabeiras, Vitória, Brazil.

Open Access   

Published:  Mar 30, 2018

DOI: 10.7324/JAPS.2018.8317
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Abstract

Myrciaria strigipes O. Berg (Myrtaceae) is a Brazilian native species, endemic to Bahia and Espírito Santo, and known popularly as “cambucá da praia”. This study aimed to evaluate the chemical profile of ethanol extract of leaves from M. strigipes (EEF), and the two in vitro activities: antioxidant and inhibition of α-glucosidase. The column chromatography separation gave friedelin (1), glutinol (2), β-sitosterol (3), 28-hydroxyfriedelin (4), and ursolic acid (5). The chromatographic profile of EEF by HPLC (354 nm) allowed the identification of three phenolic compounds: ellagic acid (6), hyperoside (7), and isoquercitrin (8). The extract EEF showed antioxidant activity in both models DPPH and ABTS, with EC50 of 61.79 ± 2.97 and 23.72 ± 0.48 μg/mL, respectively. Trolox, used as a standard, showed EC50 of 4.60 ± 0.82 and 1.08 ± 0.05 μg/mL, respectively. The obtained result of the inhibition assay on the enzyme activity of α-glucosidase revealed that EEF had an EC50 value of 40.66 ± 0.81 μg/mL, close to the positive standard deoxynojirimycin (EC50 = 37.30 ± 4.41 μg/mL). Therefore, this study contributed to the knowledge of the chemical and biological properties of M. strigipes and will subsidize future studies of this species.


Keyword:     Myrciaria strigipes HPLC Phenolic compounds Triterpenes Antioxidant activity α-glucosidase.

Citation:

Faitanin RD, Gomes JVD, Rodrigues PM, de Menezes LFT, Neto ÁC, Gonçalves RCR, Kitagawa RR, Silveira D, Jamal CM. Chemical study and evaluation of antioxidant activity and α-glucosidase inhibition of Myrciaria strigipes O. Berg (Myrtaceae). J App Pharm Sci, 2018; 8(03): 120-125.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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