Research Article | Volume: 4, Issue: 7, July, 2014

Pentacyclic triterpenes and crude extracts with antimicrobial activity from Cameroonian brown propolis samples

Sakava Paul,  Talla Emmanuel,  Chelea Matchawe,  Tchinda Tiabou Alembert,  Zeuko’o Menkem Elisabeth,  Laurent Sophie,  Vander Elst Luce,  Tagatsing Fotsing Maurice,  Yaya Gbaweng Abel Joel,  Atchade De Théodore Alex and Mbafor Tanyi Joseph   
Sakava Paul1, Talla Emmanuel2, Chelea Matchawe3, Tchinda Tiabou Alembert3, Zeuko’o Menkem Elisabeth4, Laurent Sophie5, Vander Elst Luce5, Tagatsing Fotsing Maurice6, Yaya Gbaweng Abel Joel3, Atchade De Théodore Alex 6 and Mbafor Tanyi Joseph6

1Department of Chemistry, Higher Teachers’ Training College, University of Bamenda, P.O. Box 39 Bambili, Cameroon.

2Department of Chemistry, Faculty of Science, University of Ngaoundéré, P.O. Box 454 Ngaoundéré, Cameroon.

3Institute of Medical Research and Medicinal Plants Studies (IMPM), Ministry of the Scientific Research and innovation, P. O.Box 6163 Yaounde, Cameroon.

4Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812 Yaoundé, Cameroon.

5Department of General, Organic and Biomedical Chemistry, Faculty of Medicine and Pharmacy, University of Mons, NMR and Molecular Imaging Laboratory, B-7000 Mons, Belgium.

6Department of Organic Chemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812 Yaoundé, Cameroon.

Open Access   

Published:  Jul 28, 2017

DOI: 10.7324/JAPS.2014.40701
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Abstract

The aim of this study was to isolate compounds from Cameroonian propolis extracts and to test their activities against bacteria isolated from carcass at the Yaoundé slaughterhouse. The n-hexane, ethyl acetate and ethanol extracts of propolis samples from Ngaoundal and Tala-Mokolo were separated by successive silica gel column chromatography to give six triterpenes. Their structures were determined as 25-cyclopropyl-3β-hydroxyurs-12-ene (7), cycloart-3β-hydroxy-12, 25(26)-diene (8), lup-20(29)-en-3-one (9), olean-12-en-3β, 28-diol (10), lup-20(29)-en-3β-oate (13) and 3β-hydroxylup-20(29)-ene (14). Compounds 7 and 8 were new triterpene derivatives while 10 and 13 were isolated for the first time from propolis. The structures of all the compounds were established on the basis of spectroscopic analysis. Phytochemical screening of the methanol extract (5) revealed the presence of alkaloids, reducing compounds, coumarins, saponins and tannins accounting for its broad spectrum antibacterial activities. The six isolated compounds and crude extracts were tested for antimicrobial activity against some Gram negative bacteria. The methanol extract (5) of propolis samples was active against Escherichia coli and Pseudomonas aeruginosa (MIC: 0.2 mg/ml) whereas the isolated compounds 7, 8 and 10 exclusively exhibited antimicrobial activity against Salmonellas pp (MIC: 0.1-0.15 mg/ml). The MIC values of all the four propolis products were greater than that of the standard drug (Amoxicillin): 0.1-0.2 mg/ml versus 0.4 mg/ml. Nevertheless, further pharmacological and toxicity studies on experimental animals are necessary to establish the safety of the propolis products for its use as topical antimicrobial agents.


Keyword:     Cameroonian propolis crude extracts isolation triterpenes phytochemical screening antimicrobial activity.

Citation:

SAKAVA Paul, TALLA Emmanuel, CHELEA Matchawe, TCHINDA TCHINDA Alembert., ZEUKO’O5 Mamkem Elisabeth, TAGATSING Maurice, ATCHADE De Teodore Alex, YAYA Abel Gbaweng Joel. Triterpenes and crude extracts with antimicrobial activity from Cameroonian brown propolis samples. J App Pharm Sci, 2014; 4 (07): 001-009.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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