Research Article | Volume: 8, Issue: 3, March, 2018
Research Article | Volume: 8, Issue: 3, March, 2018
β-methylcholanthrene (MC) is a potent toxic agent that related to polycyclic aromatic hydrocarbons and its adverse influence on detoxification function of chick embryo brain glutathione S-transferases (GSTs) and brain degenerative changes was investigated. MC (2 ppm/10 μl) was injected in coconut oil up to 6 ppm though air sac on 15th day for 24 hours interval and 12th day for 48 hours interval. Brain tissues were collected on 18th day post injection for experimentation. Results revealed that GST specific activity has increased significantly (p < 0.05) with model substrates by 2 ppm and 4 ppm MC in both intervals, but decreased significantly (p < 0.05) by 6 ppm in both intervals and western blot studies also shows similar pattern of GST expression. At histological level that the cerebellar layers degeneration, molecular layer expansion towards granular layer and vacuolation were observed in 24 hours interval, but severe cerebral haemorrhage and vacuolation were observed in 48 hours interval by 6 ppm MC. In conclusion, MC showed adverse influence on GSTs function and brain degeneration in chick embryo.
Dasari S, Gonuguntla S, Meriga B, Kedam T. Adverse influence of β-methylcholanthrene on detoxification function of chick embryo brain glutathione S-transferases and degenerative changes of brain. J App Pharm Sci, 2018; 8(03): 111-119.
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