Research Article | Volume: 8, Issue: 3, March, 2018

Effect of Piperine on Pharmacokinetics of Rifampicin and Isoniazid: Development and Validation of High Performance Liquid Chromatography Method

Amit Singh,  Smita Verma,  Nouh M H Al Jarari,  Ajay Pal Singh,  Neeraj Kumar Fuloria,  Shivkanya Fuloria,  Pradeep Kumar Sharma,  Chhotelal   
Amit Singh1, Smita Verma2, Nouh M H Al Jarari3, Ajay Pal Singh4, Neeraj Kumar Fuloria5, Shivkanya Fuloria5, Pradeep Kumar Sharma6, Chhotelal6

1School of Pharmacy, Monad University, Hapur, Uttar Pradesh, 245101, India.

2Department of Chemistry, National Institute of Pharmaceutical Education and Research (NIPER), Kolkata, West Bengal, India.

3Department of Pharmacology, Faculty of Medicine, University of Benghazi, Libya.

4HIMT College of Pharmacy, Knowledge Park, Phase-II, Greater Noida, Gautam Budh Nagar, Uttar Pradesh, India.

5Department of Pharmaceutical Chemistry, Faculty of Pharmacy, AIMST University, Semeling Campus, Jalan Bedong-Semeling, Bedong, Kedah Darul Aman, 08100, Malaysia.

6R. V. Northland Institute, Dadri, Gautam Budh Nagar, Uttar Pradesh, 203 207, India.

Open Access   

Published:  Mar 30, 2018

DOI: 10.7324/JAPS.2018.8311
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Abstract

In the present investigation a method for simultaneous determination of rifampicin, isoniazid and piperine by liquid chromatography has been developed and optimized in terms of specificity, accuracy, precision, sensitivity and stability. The purpose of this investigation concerns the effect of piperine on the pharmacokinetic of rifampicin and isoniazid in rat model. Suspension of rifampicin, isoniazid and piperine were administered orally in Swiss albino rat and the drug concentration in plasma was estimated by high performance liquid chromatography. The chromatic separation of rifampicin, isoniazid and piperine were achieved by carrying out on a Zorbax Eclipse XDP C18 column (150 mm × 4.6 mm × 5 μm) using potassium dihydrogen phosphate (pH 4.5) and acetonitrile as the mobile phase (30:70) at a flow rate of 0.8 mL/min with detection at 270 nm. After oral administration the observed pharmacokinetic parameter of rifampicin along with piperine indicates significant enhancement in Cmax and area under the curve (AUC), while in isoniazid with piperine shows reduced Cmax and enhanced-AUC. The observed pharmacokinetics data after oral administration of rifampicin along with piperine indicate significant enhancement in Cmax and AUC and thus bioavailability. The bioavailability of isoniazid was reduced when it was co-administered with piperine, it could be due to delay in gastric emptying time.


Keyword:     Pharmacokinetic Rifam¬picin Isoniazid Piperine Bioavailability Reverse phase-High performance liquid chromatography (RP-HPLC).

Citation:

Singh A, Verma S, Al Jarari NMH, Singh AP, Fuloria NK, Fuloria S, Sharma PK, Chhotelal. Effect of Piperine on Pharmacokinetics of Rifampicin and Isoniazid: Development and Validation of High Performance Liquid Chromatography Method. J App Pharm Sci, 2018; 8(03): 072-081.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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