Research Article | Volume: 8, Issue: 3, March, 2018

Antibiofilm activity and molecular docking studies of bioactive secondary metabolites from endophytic fungus Aspergillus nidulans on oral Candida albicans

S. S. Meenambiga K. Rajagopal   

Open Access   

Published:  Mar 30, 2018

DOI: 10.7324/JAPS.2018.8306
Abstract

Aspergillus nidulans, an endophytic fungus isolated from the medicinal plant Acacia nilotica showed potent antifungal activity against oral pathogenic strains of Candida albicans. Biofilm forming C. albicans was isolated and tested for its susceptibility to various solvent extracts of A. nidulans using biofilm inhibition assay. Gas chromatography-mass spectrometry (GC-MS) analysis was performed to identify the potential compounds in the endophytic fungal extract and mode of interaction of these compounds with the target enzyme of C. albicans was revealed using molecular docking analysis. All the solvent extracts showed good inhibitory activity on the biofilm forming C. albicans strain with maximum activity in the chloroform extract. The percentage reduction in biofilm inhibition for chloroform, ethyl acetate, hexane and methanol extracts were 74.86%, 72.53%, 60.61% and 52.60% respectively. GC-MS analysis of chloroform extract revealed the presence of flavonoids, fatty acids, amides etc. of which the flavonoids-saltillin, taxifolin and 6-methoxyflavone showed good binding interaction with the C. albicans growth regulator N-myristoyltransferase (NMT). The bioactive compounds present in A. nidulans not only inhibit C. albicans growth but also controls the biofilm formation thereby reducing the virulence of C. albicans.


Keyword:     Endophytic fungi biofilmGC-MS flavonoidsmolecular docking.


Citation:

Meenambiga SS, Rajagopal K. Antibiofilm activity and molecular docking studies of bioactive secondary metabolites from endophytic fungus Aspergillus nidulans on oral Candida albicans. J App Pharm Sci, 2018; 8(03): 037-045.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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