Research Article | Volume: 8, Issue: 2, February, 2018

Induction of novel mutants of Streptomyces lincolnensis with high Lincomycin production

Ahmed El-Sherbini A. A. Khattab   

Open Access   

Published:  Feb 27, 2018

DOI: 10.7324/JAPS.2018.8220
Abstract

Different mutants of Streptomyces lincolnensis were selected after mutagenesis and screened for Lincomycin production. Out of 60 obtained mutants after ultra-violet (UV) irradiation, 34 of these exhibited higher Lincomycin production (from 7.69 to 84.62%) than wild type strain. Through mutagenesis by ethyl methan sulfonate (EMS), out of 60 obtained mutants after EMS treatment 48 of these exhibited higher Lincomycin production (from 7.69 to 146.15%) than original strain. The superior mutant M-40-18 produced Lincomycin 146.15% higher than original wild strain. Furthermore, the random amplified polymorphic DNA (RAPD) protocol by polymerase chain reaction (PCR) machine on some selected superior mutants lead to correlate the genetic characteristics of these mutants with the results obtained from the Lincomycin productivities in comparison with the original strain. The variations in RAPD profiles confirmed the occurrence of genetic variation induced in S. lincolnensis genotype after mutagenesis.


Keyword:     Improvement LincomycinS. lincolnensis MutantsRAPD-PCR.


Citation:

El-Sherbini A and Khattab AA. Induction of novel mutants of Streptomyces lincolnensis with high Lincomycin production. J App Pharm Sci, 2018; 8(02): 128-135.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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