Research Article | Volume: 8, Issue: 2, February, 2018

A Novel HPLC Method Validation Based on Analytical Techniques of Metoclopramide Benzamide Derivative (Metoclopramide Base) and its Determination from Solid Dispersion by Solvent Evaporation Method

Nancy Kahali Jasmina Khanam   

Open Access   

Published:  Feb 27, 2018

DOI: 10.7324/JAPS.2018.8203
Abstract

The crucial endeavor in this method is focused on the study analysing recovery and column efficiency (theoretical plate) after extraction of metoclopramide from PVP K30-Metoclopramide (3:1 w/w) solid dispersion (SD). The proposed work also presents a reversed-phase high performance liquid chromatography method for the detection of very small amount of metoclopramide base followed by different analytical techniques. In this context, it may be noted that the values of limit of detection (LOD) and limit of quantitation (LOQ) for the metoclopramide base as obtained are, 0.052 μg/mL and 0.159 μg/mL respectively. The linearity is performed in the range of 2-20 μg/ mL; performance of column remained satisfactory throughout the analysis, N = 4927-2434. The percentage recovery of pure and extracted metoclopramide ranges from 103-105% and 86-120% approximately. The results collectively demonstrate that the proposed method is selective. Clear isolation of metoclopramide from SD is achieved without any solid state interference.


Keyword:     HPLC metoclopramidebase extraction solid dispersionvalidation.


Citation:

Kahali N, Khanam J. A Novel HPLC Method Validation Based on Analytical Techniques of Metoclopramide Benzamide Derivative (Metoclopramide Base) and its determination from Solid Dispersion by Solvent Evaporation Method. J App Pharm Sci, 2018; 8(02): 018-026.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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