Research Article | Volume: 7, Issue: 10, October, 2017

Alpha-Glucosidase Inhibitory Activity of Garcinia lateriflora Blume Leaves

Putu Gita Maya Widyaswari Mahayasih Berna Elya Muhammad Hanafi   

Open Access   

Published:  Oct 30, 2017

DOI: 10.7324/JAPS.2017.71014
Abstract

Garcinia lateriflora Blume., that belongs to the genus Garcinia, is known to contain polyphenol compounds that are likely to inhibit alpha-glucosidase in the treatment of diabetes mellitus. Therefore, this study aimed to evaluate and acquire the most biologically active fraction from leaves of G. lateriflora in inhibiting alpha-glucosidase. The separation of the active fraction was conducted using column chromatography and identified by thin layer chromatography. Alpha-glucosidase inhibitory activity test carried out in vitro using spectrophotometric method with p-nitrophenyl-α-D-glucopiranoside as the substrate. 17 and 12 fractions were obtained from ethyl acetate and methanol extracts, respectively. Fraction 13 (EA13) of ethyl acetate extract and fraction 10 (Me10) of methanol extract were showed the highest percent inhibition compared with the other fraction with IC50 values 8.96 µg/ml and 18.52 µg/ml, respectively. While the IC50 value for Acarbose is 39.53 µg/ml. Furthermore, fraction EA13 is the most active fraction in inhibiting alpha-glucosidase compared with the extract, other fractions, and Acarbose.


Keyword:     alpha-glucosidase column chromatography Diabetes mellitus Garcinia laterifloraleaves in vitro assay.


Citation:

Mahayasih PGM, Elya B, Hanafi M. Alpha-Glucosidase Inhibitory Activity of Garcinia lateriflora Blume Leaves. J App Pharm Sci, 2017; 7 (10): 100-104.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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