Research Article | Volume: 7, Issue: 8, August, 2017

Green Synthesis of Silver Nanoparticles from Caesalpinia gilliesii (Hook) leaves: antimicrobial activity and in vitro cytotoxic effect against BJ-1 and MCF-7 cells

Mahmoud Emam,  Mohamed A. El Raey,  Wael H. Eisa,  Alaadin E. El- Haddad,  Samir M. Osman,  Mohamed A. El-Ansari,  Abdel-Gawad M. Rabie   
Mahmoud Emam1, Mohamed A. El Raey1, Wael H. Eisa2, Alaadin E. El- Haddad3, Samir M. Osman3, Mohamed A. El-Ansari1, Abdel-Gawad M. Rabie4

1Department of Phytochemistry and Plant Systematics, National Research Centre, Dokki, Cairo, Egypt.

2Spectroscopy Department, Physics Division, National Research Centre, Dokki, Cairo, Egypt.

3Department of Pharmacognosy, Faculty of Pharmacy, October 6 University, Giza, Egypt.

4Department of Chemistry, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt.

Open Access   

Published:  Aug 30, 2017

DOI: 10.7324/JAPS.2017.70831
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Abstract

Green synthesis of silver nanoparticles using Caesalpinia gilliesii (Hook) leaves extract (70% MeOH) for the first time as a reducing agent were investigated for their antimicrobial and cytotoxic activity (using the MTT assay). After exposing the oxidizing agent of silver ions to C. gilliesii leaves extract, the rapid reduction in the solution is observed due to the construction of silver nanoparticles. The prepared nanoparticles were elucidated by using UV-visible spectroscopy, Fourier Transforms infrared spectroscopy (FT-IR) and transmission electron microscope (TEM). The purified silver nanoparticles demonstrated promising antimicrobial activity against tested pathogens than hydroalcoholic extract. Cell viability by using the MTT assay demonstrated cytotoxic activity of the synthesized Ag NPs with C. gilliesii against normal skin fibroblast (BJ-1) and human breast cancer cell (MCF-7) with IC50= 80.1 and 36.5 𝜇g/mL at 48 hours incubation, respectively. Depending on the phenolic and flavonoid contents, C. gilliesii could be used for simple, nonhazardous, eco-friendly, cost-effective and efficient synthesis of Ag NPs that can be applied into medicinal field.


Keyword:     Silver nanoparticlesTotal phenolic & flavonoidAntimicrobialAnticancerCaesalpinia gilliesii.

Citation:

Emam M, El-Raey MA, Eisa WH, El-Haddad AE, Osman SM, ElAnsari MA, Rabie AM. Antimicrobial of Green Synthesis Silver Nanoparticles Using Extract of Caesalpinia gilliesii (Hook) leaves: and in Vitro Cytotoxic Effect against BJ1 and MCF-7 Cells. J App Pharm Sci, 2017; 7 (08): 226-233.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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