Research Article | Volume: 7, Issue: 5, May, 2017

Determination of phenolic profile by HPLC-ESI-MS/MS and antibacterial activity of Eugenia platysema against mollicutes strains

Adrielli Tenfen Diogo Alexandre Siebert Daniel Spudeit Caio Maurício Mendes de Cordova Gustavo Amadeu Micke Michele Debiasi Alberton   

Open Access   

Published:  May 30, 2017

DOI: 10.7324/JAPS.2017.70502

This study evaluated the phenolic composition of the extract and fractions from E. platysema leaves, in addition to antibacterial activity against different types of bacteria, like mollicutes and cel wall bacteria. The crude extract (CHE) was partitioned with solvents to yield fractions of different polarities (dichloromethane, ethyl acetate, butanol and water). The chemical composition of CHE and its fractions were evaluated by HPLC-LC-ESI-MS/MS together with 76 standard phenolic compounds. Nine known compounds were identified for the first time from this species: hydroxybenzoic-O-hexoside acid [1], gallocatechin [2], syringic acid [3], 4-hydroxycinammic acid [4], salycilic acid [5], isoquercetin [6], isorhamnetin [7], luteolin-3’-O-glucuronide [8] and luteolin [9]. The evaluation of antibacterial activity was performed using the broth dilution method. The CHE and fractions of E. platysema were active against mollicutes strains, being the best result presented by the dichloromethane fraction against U. urealyticum, M. mycoides subsp. Capri and M. hominis (125 µg mL-1).

Keyword:     Eugenia platysema phenolic compounds antibacterial activity mollicutes.


Tenfen A, Siebert DA, Spudeit D, de Cordova CMM, Micke GA, Alberton MD. Determination of phenolic profile by HPLC-ESIMS/MS and antibacterial activity of Eugenia platysema against mollicutes strains. J App Pharm Sci, 2017; 7 (05): 007-011.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text


Article Metrics

419 Absract views 15 PDF Downloads 434 Total views

   Abstract      Pdf Download

Related Search

By author names

Citiaion Alert By Google Scholar

Name Required
Email Required Invalid Email Address

Comment required