Research Article | Volume: 7, Issue : 4, April, 2017

Phytochemical study and anti-inflammatory effect of Psychotria stenocalyx (Rubiaceae)

Gustavo Silva Queiroz,  Ana Beatriz Gobbo Luz,  Marcus Vinícius Pereira dos Santos Nascimento,  Sergio Scherrer Thomasi,  Antonio Gilberto Ferreira,  Eduardo Monguilhott Dalmarco,  Ines Maria Costa Brighente   
Gustavo Silva Queiroz1, Ana Beatriz Gobbo Luz2, Marcus Vinícius Pereira dos Santos Nascimento2, Sergio Scherrer Thomasi3, Antonio Gilberto Ferreira4, Eduardo Monguilhott Dalmarco2, Ines Maria Costa Brighente1

1Department of Chemistry,Federal University of Santa Catarina, Florianópolis – SC, Brazil.

2Department of Clinical Analysis, Federal University of Santa Catarina, Florianópolis – SC, Brazil.

3Department of Chemistry, Federal University of Lavras, Lavras – MG, Brazil.

4Department of Chemistry, Federal University of São Carlos, São Carlos – SP, Brazil.

Open Access   

Published:  Apr 30, 2017

DOI: 10.7324/JAPS.2017.70425
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Abstract

In this study, an in vivo anti-inflammatory assay was conducted on Psychotria stenocalyx ethanol extract of leaves, evaluating this effect on cell migration and exudate formation in a murine carrageenan-induced model of pleurisy. The extract showed the optimum dose, at 400 mg/kg, inhibiting cellular and vascular parameters: total leucocytes due to polymorphonuclear migration and exudate formation (p < 0.01). A phytochemical study was conducted using the hyphenated techniques HPLC-DAD-SPE/NMR and HPLC-DAD-ESI-TOF-HRMS, in which seven monoterpene indole alkaloids were identified: lyaloside 1, (E)-O-(6′)-cinnamoyl-4”-hydroxy-3”,5”-dimethoxy-lyaloside 2, strictosamide 3, pauridianthoside 4, vallesiachotamine lactone 5, E-vallesiachotamine 6 and Z-vallesiachotamine 7, in the alkaloid fraction, obtained by an acid-base extraction on P. stenocalyx crude extract. This is the first study developed with this species.


Keyword:     Psychotria stenocalyx anti-inflammatory monoterpene indole alkaloids HPLC-DAD-SPE/NMR.

Citation:

Queiroz GS, Luz ABG, Nascimento MVPDS, Thomasi SS, Ferreira AG , Dalmarco EM, Brighente IMC. Phytochemical study and antiinflammatory effect of Psychotria stenocalyx (Rubiaceae). J App Pharm Sci, 2017; 7 (04): 168-173.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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