Research Article | Volume: 7, Issue : 4, April, 2017

Endophytes isolated from passion fruit plants: molecular identification, chemical characterization and antibacterial activity of secondary metabolites

Mariana Sanches Santos Ravely Casarotti Orlandelli Julio Cesar Polonio Marcos Alessandro dos Santos Ribeiro Maria Helena Sarragiotto João Lúcio Azevedo João Alencar Pamphile   

Open Access   

Published:  Apr 30, 2017

DOI: 10.7324/JAPS.2017.70405
Abstract

Endophytic fungi inhabit the interior of healthy plants without any apparent harm and may synthesize bioactive compounds, which constitute an alternative for the control of human pathogens. Current study reports the molecular identification of two endophytes from passion fruit plants: isolate PE1-13 (from Passiflora edulis f. flavicarpa) has been identified as Phyllosticta sp. and isolate PA8-2 (from Passiflora alata) is a Cercospora beticola strain. Thin layer chromatography showed that steroids and triterpenoids were present in the secondary metabolites produced by the two endophytes. The antibacterial assay against Enterococcus faecalis, Salmonella typhi and Streptococcus pyogenes indicated that the fungal metabolites reduced up to 98.1±0.5% of bacterial growth. Results suggest that these endophytes are capable of producing antibacterial compounds.


Keyword:     Endophytic fungi antimicrobial action Passiflora genus rDNA sequencing thin layer chromatography.


Citation:

Santos MS, Orlandelli RC, Polonio JC, Ribeiro MAS, Sarragiotto MH, Azevedo JL, Pamphile JA. Endophytes isolated from passion fruit plants: molecular identification, chemical characterization and antibacterial activity of secondary metabolites. J App Pharm Sci, 2017; 7 (04): 038-043.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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