Research Article | Volume: 7, Issue: 2, February, 2017

Evaluation of the cytotoxic activity of extracts from six species of Phlomis genus

Parisa Sarkhail,  Shamim Sahranavard ,  ,  Marjan Nikan,  Saeedeh Gafari,  Bahara Eslami-Tehrani   
Parisa Sarkhail1, Shamim Sahranavard2 , 4, Marjan Nikan3, Saeedeh Gafari4, Bahara Eslami-Tehrani4

1Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran.

2Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

3Medicinal Plants Research Center, Tehran University of Medical Sciences, Tehran, Iran.

4Traditional Medicine and Material Medical Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Open Access   

Published:  Feb 27, 2017

DOI: 10.7324/JAPS.2017.70225
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Abstract

The present study was designed to investigate the cytotoxic activity of the total extract (methanol 80%) and fractions from six Phlomis species, including P. anisodontea, P. bruguieri, P. caucasica, P. olivieri, P. persica and P. kurdica against four human cancer cell lines (HepG2, MCF7, HT29 and A549) and one normal cell line (MDBK). The in vitro cytotoxicity of different concentrations (31.25 - 1000μg/mL) of the extracts and fractions evaluated by the MTT assay. Data revealed that all six extracts exhibited cytotoxic activity against at least one cancer cell line. The interesting cytotoxic activity on MCF7 cell line, respectively, was observed for dichloromethane fractions of P. anisodontea and P. Kurdica, and ethylacetate fractions of P. kurdica, P. anisodontea and P. bruguieri. In addition, the highest cytotoxic activity against A549 cells was found for ethylacetate fraction from P. caucasica and P. kurdica. Overall, the results of this study suggest that among selected plants, P. kurdica, P. anisodontea and P. caucasica are the best candidates for further investigations on the mechanisms of cytotoxic action and isolated the active compounds.


Keyword:     Phlomis species cytotoxicity MTT assay.

Citation:

Sarkhail P, Sahranavard S, Nikan M, Gafari S, Eslami-Tehrani B. Evaluation of the cytotoxic activity of extracts from six species of phlomis genus. J App Pharm Sci, 2017; 7 (02): 180-184.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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