Research Article | Volume: 7, Issue: 5, May, 2017
Screening of in vitro cytotoxic activity of brown seaweeds against hepatocellular carcinoma
Research Article | Volume: 7, Issue: 5, May, 2017
Screening of in vitro cytotoxic activity of brown seaweeds against hepatocellular carcinoma
This study attempts to screen the potential chemotherapeutic agents from three seaweeds extracted with methanol and tested against hepatocellular carcinoma cells (Hep3B) and normal cell line (Vero). Using MTT assay, the extracts were checked for their cytotoxicity at six different concentrations. The study exhibited decrease of the cell viability percentage in dose dependent manner as signified by cell death. Sargassum wightii extract shows potential cytotoxic activity with CTC50 of 10.02 ± 1.06 and 180.65 ± 2.87µg/ml against Vero cells and Hep3B respectively. The methanol fraction led to cell shrinkage, plasma membrane damage and apoptotic bodies formation showing bioactive components as profound influencing factors for anticancer effects. Further research need to be explored to purify and characterize the methanol extract to identify the potent therapeutic tool against liver cancer.
Suja G, Vinothkumar T, Lakshmanasenthil S, Yesudas R, Chacko A, Suganya P. Screening of in vitro cytotoxic activity of brown seaweeds against hepatocellular carcinoma. J App Pharm Sci, 2017; 7 (05): 051-055.