Research Article | Volume: 7, Issue: 5, May, 2017

Screening of in vitro cytotoxic activity of brown seaweeds against hepatocellular carcinoma

Suja Gunasekaran Vinoth Kumar Thirumalairaj Lakshmana Senthil Shanmugasokan Suganya Pitchai Rincy Yesudas Amrutha Chacko   

Open Access   

Published:  May 30, 2017

DOI: 10.7324/JAPS.2017.70509
Abstract

This study attempts to screen the potential chemotherapeutic agents from three seaweeds extracted with methanol and tested against hepatocellular carcinoma cells (Hep3B) and normal cell line (Vero). Using MTT assay, the extracts were checked for their cytotoxicity at six different concentrations. The study exhibited decrease of the cell viability percentage in dose dependent manner as signified by cell death. Sargassum wightii extract shows potential cytotoxic activity with CTC50 of 10.02 ± 1.06 and 180.65 ± 2.87µg/ml against Vero cells and Hep3B respectively. The methanol fraction led to cell shrinkage, plasma membrane damage and apoptotic bodies formation showing bioactive components as profound influencing factors for anticancer effects. Further research need to be explored to purify and characterize the methanol extract to identify the potent therapeutic tool against liver cancer.


Keyword:     Cancer Seaweeds Sargassum wightii Cytotoxicity MTT assay.


Citation:

Suja G, Vinothkumar T, Lakshmanasenthil S, Yesudas R, Chacko A, Suganya P. Screening of in vitro cytotoxic activity of brown seaweeds against hepatocellular carcinoma. J App Pharm Sci, 2017; 7 (05): 051-055.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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