Published:  Feb 27, 2017DOI: 10.7324/JAPS.2017.70204
The current study aimed to determine the antioxidant compounds in Solanum nigrum (S. nigrum) leaves extract, to synthesize silver nanoparticles (AgNPs) and to evaluate the protective role of the extract against the hepatotoxicity and genotoxicity of AgNPs compared to CCl4 in rats. Eight groups of female Sprague-Dawley rats were treated orally for 3 weeks included the control group, CCl4-trared group (0.1 ml/kg b.w twice a week), AgNPs-treated group (50 mg/kg b.w/day), AgNPs plus CCl4-treated group, S. nigrum leaves extract-treated group (0.5 mg/kg b.w) and the groups treated with AgNPs and/or CCl4 plus the extract. The results indicated that the extract was rich in the total phenolic, flavoniods and β-carotene. The size of synthesized AgNPs was 30-50 nm. Administration of AgNPs and/or CCl4 resulted in severe hepatotoxicity and histological changes, increased DNA fragmentation and down regulation of antioxidant gene expression in liver. The extract was safe and succeeded to mitigate the hazards effect of AgNPs and/or CCl4. It could be concluded that AgNPs have toxic effects and caution should be taken when they use in food or medical application. S. nigrum extract succeeded to protect the liver due to its higher content of antioxidant compounds.
Abdel-Wahhab MA, Ahmed HMS, Abdel-Aziem SH, El-Nekeety AA, Abdel-Kader HAM, Abdel-Aziz MS, Sharaf HA, Naguib HM, El-Denshary ES. Modulation of hepatotoxicity, DNA fragmentation and gene expression of Solanum nigrum leaves extract in rats treated with silver nanoparticles. J App Pharm Sci, 2017; 7 (02): 025-035.
427 Absract views 11 PDF Downloads 438 Total views
Antimicrobial Activity of Extracellularly Synthesized Silver Nanoparticles using Lactobacillus Species Obtained from VIZYLAC CapsulePratik R. Chaudhari,Shalaka A. Masurkar, Vrishali B. Shidore, Suresh P. Kamble
Degalactotigonin induces cytotoxicity and cell cycle arrest in triple negative breast cancer cells (MDA MB 231)D. Hamsa, K. Swathi, Sri Renukadevi Balusamy, S. Sumathi
Comparative effect of chronic administration of chloroquine phosphate and methanol extract of Landolphia owariensis on the activities of rat EnzymesIlesanmi F F, Balogun E A, Ilesanmi O S
Protective effect of curcumin on oxidative stress and DNA fragmentation against lambda cyhalothrin-induced liver damage in rats
The impact of oxidative stress on Par2, Ptgs2 genes expression in rat duodenal epithelial cells under conditions of prolonged gastric hypochlorhydria and with administration of multiprobiotic