Research Article | Volume: 7, Issue: 1, January, 2017

Biological capacity and chemical composition of secondary metabolites from representatives Japanese Lichens

Eman F. Ahmed,  Waill A. Elkhateeb ,  Hanan A. A. Taie,  Mostafa E. Rateb ,  Walid Fayad   
Eman F. Ahmed1, Waill A. Elkhateeb1 & 2, Hanan A. A. Taie3, Mostafa E. Rateb4 & 5, Walid Fayad6

1Chemistry of natural and microbial products, National Research Centre, Dokki, Giza, 12622, Egypt.

2Laboratory of Microbial Technology Department of Bioscience and Biotechnology. Faculty of Agriculture, Kyushu University.​​​​​​​

3Department of Plant Biochemistry-National Research Centre, Dokki, Cairo, Egypt.​​​​​​​

4Pharmacognosy Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, Egypt.​​​​​​​

5School of Science & Sport, University of the West of Scotland, Paisley PA1 2BE, UK.​​​​​​​

6Drug Bioassay-Cell Culture Laboratory, Pharmacognosy Department, National Research Centre, Dokki, Giza, 12622, Egypt.

Open Access   

Published:  Jan 31, 2017

DOI: 10.7324/JAPS.2017.70113
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Abstract

Lichens represent a well-known symbiotic system. Brachythecium velutinum (Brown), Brachythecium rutabulum, Lepraria incana, Brachythecium velutinum (Green) and Dirinaria applanata were collected from the tree trunks and rocks in Japanese forests. The alcoholic extracts of these lichens were examined for their antioxidant and cytotoxicity effect. Dirinaria applanata methanolic extract (2.5 mg /mL–1) was the most active in scavenging DPPH free radical (75.09±0.37%) and in reducing Fe3+/ferricyanide complex to the ferrous form (1.88±0.03). It was also the most potent in ABTS•+ scavenging activity using ABTS assay 89.35%) at a concentration (0.5 mg.mL–1). Brachythecium rutabulum and Dirinaria applanata extracts demonstrated a significant in vitro cytotoxic effect on 549 human lung carcinoma cell line with IC50 values (34.7±2.1 and 38.6±2.8) µg/mL, respectively. Dereplication of these extracts using LC-HRMS was performed to gain insight about secondary metabolism profiles of these lichens and their rule in the antioxidant and cytotoxic activities.


Keyword:     Lichens Antioxidant Cytotoxicity (LC-HRMS) Terpene Tannins.

Citation:

Ahmed EF, Elkhateeb WA, Taie HAA, Rateb ME, Fayad W. Biological capacity and chemical composition of secondary metabolites from representatives Japanese Lichens. J App Pharm Sci, 2017; 7 (01): 098-103.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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