Research Article | Volume : 1 Issue : 8, October 2011

Micropropagation and shoot regeneration of Cistus creticus ssp. Creticus

Panagiotis Madesis,  Elpiniki Konstantinidou,  Athanasios Tsaftaris,  Irini Nianiou-Obeidat   
Panagiotis Madesis2, Elpiniki Konstantinidou1, Athanasios Tsaftaris2, Irini Nianiou-Obeidat1

1Department of Genetics and Plant Breeding, School of Agriculture, Aristotle University of Thessaloniki, P.O. Box 261, Thessaloniki GR54124, Greece.

2Institute of Agrobiotechnology, CERTH, 6th km Charilaou-Thermis Road, P.O. Box 361, Thermi GR57001,Thessaloniki, Greece.Department of Genetics and Plant Breeding, School of Agriculture, Aristotle University of Thessaloniki, P.O. Box 261, Thessaloniki GR54124, Greece.

Open Access   

PDF [ 1.6 MB]
Request Permission
Share
Download Citation
Print
Download PDF
Abstract

Cistus creticus L. ssp. creticus is a medicinal aromatic shrub native in Crete (Greece). The protocol described in this paper provides optimal levels of growth regulators required to obtain high regeneration rates of Cistus in vitro. Micropropagation has been achieved through rapid proliferation of shoot-tips on Murashige and Skoog (MS) basal medium supplemented with 0.2 mg l-1 6-benzylaminopurine (BAP). After four weeks shoots were transferred to MS medium without growth regulators for further development and rooting. The highest percentage of regenerated shoots was obtained with 0.1 mg l-1 TDZ and 0.1 mg l-1 NAA after 4 weeks. Elongation and rooting was readily achieved when multiple shoots more than 1 cm in length were singled out and cultured on the MS medium without growth regulators. The plantlets were successfully adapted and grew vigorously in greenhouse conditions. This is the first report of shoot regeneration in the genus Cistus. The regeneration protocol developed in this study provides a basis for further investigation of the medicinally active constituents of this elite medicinal plant.


Keyword:     a-naphthaleneacetic acid (NAA) cretan ladano shoot regeneration thidiazuron (TDZ).

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
HTML Full Text

Reference

Article Metrics
384 Views 17 Downloads 401 Total

Year

Month

Related Search

By author names