Research Article | Volume: 6, Issue: 7, July, 2016

Antimicrobial Activity of Protein Hydrolysate from Marine Molluscs Babylonia spirata (Linnaeus, 1758)

Amutha Kuppusamy Selvakumari Ulagesan   

Open Access   

Published:  Jul 28, 2016

DOI: 10.7324/JAPS.2016.60711
Abstract

The present study is to investigate the antimicrobial activity of protein hydrolysate of marine water mollusks Babylonia spirata (Linnaeus, 1758). Protein hydrolysate was prepared from tissue of Babylonia spirata by enzymatic hydrolysis. Enzyme digestion were carried out with the enzyme Trypsin. The protein concentration was estimated by Bradford’s method and the protein quantification was done by using SDS PAGE analysis. Antibacterial assay was carried out against four bacterial pathogens by agar well diffusion method and antifungal activity was performed against three human pathogenic fungal strains. 2.6mg/ml protein concentration was estimated by Bradford’s method and 40 to 200 kDa protein bands were resulted in SDS PAGE analysis. In antimicrobial activity, the maximum zone of inhibition was observed against Staphylococcus aureus 22.16 +1.04mm at 1000µg/ml concentration and the maximum zone of inhibition was observed in Aspergillus fumigatus 13.5+0.5 in 1000µg/ml concentration. These results are signify that the protein hydrolysate of marine molluscs Babylonia spirata express remarkable antimicrobial activity.


Keyword:     Gastropods Babylonia spirata Enzymatic digestion Protein hydrolysate Antimicrobial activity.


Citation:

Kuppusamy A, Ulagesan S. Antimicrobial Activity of Protein Hydrolysate from Marine Molluscs Babylonia spirata (Linnaeus, 1758). J App Pharm Sci, 2016; 6 (07): 073-077.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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