Research Article | Volume: 6, Issue: 7, July, 2016

Development and validation of a stability indicating HPLC-diode array-fluorescence method for the determination of meclofenoxate hydrochloride and p-chlorophenoxyacetic acid

Marwa Said Moneeb Feda Elgammal Suzy Mohamed Sabry   

Open Access   

Published:  Jul 28, 2016

DOI: 10.7324/JAPS.2016.60701
Abstract

The present work deals with the development and validation of a stability indicating HPLC-diode array-fluorescence method (HPLC-DAD-FL) for the determination of meclofenoxate hydrochloride (MFX) and its hydrolytic degradation product p-chlorophenoxyacetic acid (pCPA). The chromatographic separation was achieved using a reversed phase Zorbax Eclipse SB-C18 column (250 x 4.6 mm i.d., 5 μm particle size), a mobile phase composed of 20 mM phosphate buffer, adjusted to pH 3, and acetonitrile (65: 35, v/v); and isocratic flow at a rate of 1 mL min-1.The detection was carried out using a diode array detector (DAD) at 225 nm and a fluorescence detector (FL) at λex/em, 225/310 nm. The linear dynamic ranges were found to be 0.5-100 and 0.25-100 μgmL-1 (DAD); and 0.05-20 and 0.01-8 μg mL-1 (FL) for MFX and pCPA, respectively. Detection limits were 0.05 and 0.04μgmL-1 (DAD); and 0.005 and 0.001 μg mL-1 (FL) for MFX and pCPA, respectively. The investigated method was applied to the assay of MFX in tablets and vials dosage forms and also to the kinetics stability studies of MFX in different media. Further, the dissolution rate of the tablets was examined using the proposed method.


Keyword:     Meclofenoxatep-chlorophenoxyacetic acid stability indicating HPLC kinetics Dissolution fluorimetric detection.


Citation:

Marwa Said Moneeb, Feda Elgammal, Suzy Mohamed Sabry., Development and validation of a stability indicating HPLC-diode array-fluorescence method for the determination of meclofenoxate hydrochloride and p-chlorophenoxyacetic acid. J App Pharm Sci, 2016; 6 (07): 001-011.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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