Research Article | Volume: 6, Issue: 6, June, 2016

Production of antioxidant xylooligosaccharides from lignocellulosic materials using Bacillus amyloliquifaciens NRRL B-14393 xylanase

Mona M. Rashad Abeer E. Mahmoud Mohamed U. Nooman Hadeer A. Mahmoud Alaa El-Din M.M. El-Torky Akaber T. Keshta   

Open Access   

Published:  Jun 28, 2016

DOI: 10.7324/JAPS.2016.60606
Abstract

The present study aimed to evaluate the enzymatic production of XOS, antioxidant activities together with total phenolic contents from different lignocellulosic materials and birchwoodxylan using both crude and pure forms of Bacillus amyloliquifaciens NRRL B-14393 xylanase respectively. The mode of action of the pure xylanase was studied by HPLC and the end products analysis of birchwoodxylan revealed that xylose, xylobiose and xylotriose were the only end products. The resulted XOS mixture exhibited potent antioxidant activity of 87.36 % and high total phenolic content of 182.88 mg GAE/ liter of extract. XOS were obtained from different alkali pretreated agricultural residues by the crude xylanase action with a yield in a range from 54.4% to85.5%. Maximum scavenging abilities and total phenolic contents of XOS mixtures of all the tested materials were determined. XOS mixtures of orange and mango peels revealed relatively higher antioxidant activities (96% and 76.84%, respectively) and total phenolic acid contents (156.32 and 133.74 mg GAE/ liter of extract, respectively) compared to the other XOS mixtures. In conclusion, Bacillus amyloliquifaciens NRRL B-14393 xylanase could be a promising source for production of XOS with high phenolic contents and antioxidant activities.


Keyword:     Xylanase xylooligosaccharides antioxidantlignocellulosic materialsBacillus amyloliquifaciens NRRL B-14393.


Citation:

Rashad MM, Mahmoud AE, Nooman MU, Mahmoud HA, ElTorky AEM, Keshta AT. Production of antioxidant xylooligosaccharides from lignocellulosic materials using Bacillus amyloliquifaciens NRRL B-14393 xylanase. J App Pharm Sci, 2016; 6 (06): 030-036

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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