Research Article | Volume : 1 Issue : 7, September 2011

Development and validation of spectrofluorimetric method for estimation of deflazacort in tablets

Patel Satish A. Patel Natvarlal J.   

Open Access   

Abstract

AbstractA simple and sensitive spectrofluorimetric method has been developed for the determination of deflazacort in pharmaceutical tablet dosage forms. The method was based on the liebermann-burchard reaction, in which the chloroform extract of deflazacort is reacted with acetic anhydride and sulfuric acid to produce strong fluorescence. The resulting fluorophor exhibit excitation and emission wavelengths at 300 and 435 nm, respectively. Linear relationship for the fluorescence intensity was obtained in the concentration range of 0.5 - 10 µg/ml. The method was validated in terms of linearity (0.5-10 µg/ml), repeatability (RSD, 0.99 %), precision (intra-day variation, RSD, 0.239 to 1.287 % and inter-day variation, RSD, 0.360 to 1.830 %) and accuracy (99.12 to 100.28 %). The limit of detection and limit of quantification for deflazacort were found to be 0.15 and 0.45 85.70 µg/ml, respectively. The developed method was successfully used for the assay of deflazacort tablet formulation. The spectrofluorimetric method was found to be simple, sensitive, accurate, precise and economic and can be used for the routine quality control testing of deflazacort in tablet dosage form.


Keyword:     Deflazacort spectrofluorimetric liebermann-burchard reaction fluorophor validation tablet.


Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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