AbstractA simple and sensitive spectrofluorimetric method has been developed for the determination of deflazacort in pharmaceutical tablet dosage forms. The method was based on the liebermann-burchard reaction, in which the chloroform extract of deflazacort is reacted with acetic anhydride and sulfuric acid to produce strong fluorescence. The resulting fluorophor exhibit excitation and emission wavelengths at 300 and 435 nm, respectively. Linear relationship for the fluorescence intensity was obtained in the concentration range of 0.5 - 10 µg/ml. The method was validated in terms of linearity (0.5-10 µg/ml), repeatability (RSD, 0.99 %), precision (intra-day variation, RSD, 0.239 to 1.287 % and inter-day variation, RSD, 0.360 to 1.830 %) and accuracy (99.12 to 100.28 %). The limit of detection and limit of quantification for deflazacort were found to be 0.15 and 0.45 85.70 µg/ml, respectively. The developed method was successfully used for the assay of deflazacort tablet formulation. The spectrofluorimetric method was found to be simple, sensitive, accurate, precise and economic and can be used for the routine quality control testing of deflazacort in tablet dosage form.
332 Absract views 6 PDF Downloads 338 Total views
High performance thin layer chromatographic method for estimation of deflazacort in tabletPatel Satish A, Patel Natvarlal J.
Development and validation of dissolution proceduresBhavesh Vaghela, Rajan Kayastha, Nayana Bhatt, Nimish Pathak, Dashrath Rathod
Development of pH-independent matrix type sustained release drug delivery system of propranolol hydrochlorideHaresh T Mulani, Bhumin Patel, Nehal J Shah