Research Article | Volume: 6, Issue: 5, May, 2016

Evaluation of In vitro Antioxidant and In vivo Pharmacological Activity of Leaf Extracts of Hoya parasitica (Wall.)

Ummee Habiba Tania Md Rajib Hassan Nusrat Jahan Eshita Rumana Akhter Mohammad Shahriar   

Open Access   

Published:  May 28, 2016

DOI: 10.7324/JAPS.2016.60526
Abstract

In present study, leaf extract of Hoya parasitica Wall. was evaluated for in vitro antioxidant and membrane stabilizing activity along with in vivo gastro intestinal motility and acute toxicity. Five different assays were performed to evaluate antioxidant activity. In DPPH free radical scavenging activity, methanol, ethanol and chloroform extract exhibited IC50 value similar to standard ascorbic acid. The presence of flavonoid and phenolic contents was also similar in all the plant extracts. However, chloroform extract showed remarkable reducing power capacity (69.10% at 200µg/mL). In case of membrane stabilization, the chloroform extract showed maximum inhibition (32.62 %) of haemolysis, whereas the ethanol extract showed a significant (p<0.001) human RBC membrane stabilizing effect. In vivo gastrointestinal motility test indicates significant (p<0.001) increase in gastrointestinal motility by Methanol extract (100 and 200 mg/kg b.w.) and ethanol extract (200 mg/kg b.w.) compared to standard. Highest dose introduced as 1000, 2000 and 3000 mg/kg body weight of each extracts in acute toxicity study and did not shown any sign of toxicity in Swiss albino mice. The result obtained from this study, can be considered as preliminary and further sophisticated investigation is needed to isolate new bioactive compounds that might act as led compounds in future.


Keyword:     Hoya parasitica Membrane stabilizing In vitro antioxidant activity IC50 toxicity GI motility.


Citation:

Tania UH, Hassan MR, Eshita NJ, Akhter R, Shahriar M. Evaluation of In vitro Antioxidant and In vivo Pharmacological Activity of Leaf Extracts of Hoya parasitica (Wall.). J App Pharm Sci, 2016; 6 (05): 163-170.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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