Research Article | Volume : 1 Issue : 7, September 2011

Quantification of Colchicine in Seed and Tuber samples of Gloriosa superba by High Performance Liquid Chromatography method

Kavina .J,  R.Gopi,  R.Panneerselvam   
Kavina .J, R.Gopi, R.Panneerselvam

Stress physiology lab, Department of Botany, Annamalai University, Annamalai nagar – 608 002 Tamil Nadu, INDIA.

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Abstract

AbstractTo quantify the colchicine (alkaloid) present in Gloriosa superba by High Performance Liquid Chromatography (HPLC) method. Gloriosa superba species are known for their content of colchicine, a valuable alkaloid in the seeds and tubers (corms). HPLC analysis was carried out to confirm the presence of colchicine in Gloriosa superba. Treatment of gibberellic acid and Psedomonas aerugenosa improved the content of colchicine in seeds and tubers. Seeds of Gloriosa superba were rich in colchicines content than the tubers sample.


Keyword:     Colchicine Gibberellic acid Gloriosa superba HPLC Psedomonas aerugenosa treatments.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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