Objective: Marine fungi play an important role in human and animal health, leading compounds to new drug discoveries and prospects for their bioactivity potential.Materials and Methods: Paecilomyces WE3-F was isolated from marine sediment (Red Sea, Shalateen, Egypt). Fungal isolate was screened for their antagonistic activity against four Gram-positive (Bacillus cereus, Lesteria monocytogenes, Micrococcus luteus and Staphylococcu aureus) and four Gram-negative (Aeromonas hydrophila, Flavobacteruim sp, Pseudomonas aeruginosa and Vibrio cholera) pathogenic bacteria. Paecilomyces WE3-F was identified using 18S rRNA technology. Seven factors were chosen to be screened for bioactivity using the Placket Burman experimental design: sucrose, yeast extract, Na NO3, temperature, initial pH, inoculum size, and incubation period. Results: Among conditional factors, acidic pH and 1.5 ml inoculum size favored the bioactive metabolites. Furthermore, a number of solvents have been experimented for the extraction of the bioactive metabolite(s). Dichloromethane (DCM) crude extract from the fermentation broth of a marine Paecilomyces WE3-F showed the highest activity with averages of 26 and 24 mm against G-ve and G+ve, respectively. Under optimal culture conditions, the maximum extractable compound concentration in a 10-L culture medium reached 83.4 mg/L. Based on data obtained by thin layer chromatogram (TLC), gas chromatography - mass spectrum (GC-MS) and Fourier Transform Infrared (FTIR) the major compound, betulin was structurally identified. Conclusions: The isolated marine Paecilomyces WE3-F, therefore, showed the ability to produce a betulin yield after optimal operating conditions for antibacterial potential.
348 Absract views 19 PDF Downloads 367 Total views
Synthesis and Antimicrobial studies of novel Benzimidazole derivatives
Leaf and Seed extracts of Bixa orellana L. exert anti-microbial activity against bacterial pathogensTamil Selvi A., Dinesh M. G., Satyan R. S., Chandrasekaran B., Rose C.