Research Article | Volume: 6, Issue: 2, February, 2016

Quinine-Loaded Polymeric Nanoparticles: Validation of a simple HPLC-PDA Method to Determine Drug Entrapment and Evaluation of its Photostability

Luana Roberta Michels Lisiane Bajerski Tamara Ramos Maciel Letícia Marques Colomé Sandra Elisa Haas   

Open Access   

Published:  Feb 27, 2016

DOI: 10.7324/JAPS.2016.60202
Abstract

Liquid chromatographic method was developed and validated for quantitative determination of quinine in polymeric nanoparticles. The method was performed using a Waters RP-18 column using a mobile phase consisting of acetonitrile:water:triethylamine (60:40:0.01 v/v/v, and pH aqueous phase adjusted to 3.0 with phosphoric acid). The flow rate was 1.0 mL min-1 and the detection was achieved with a UV-PDA set at 232 nm. The response was linear over a range of 12.0 to 24.0 µg.mL-1 (r = 0.9995). The relative standard deviation values for intra-day and inter-day precision studies were less than 2% and the accuracy was 98.8% to Nc1 and 97.3% to Nc2. The samples free of quinine and quinine-loaded polymeric nanoparticles were subjected to photodegradation conditions. A considerable reduction of degradation of quinine occurred in polymeric nanoparticles. Through these results, it was clear that the nanoencapsulation of quinine protects the drug from degradation by exposure to UV-A light. The analytical method was validated according to International Conference on Harmonization Guidelines and Center for Drug Evaluation and Research.


Keyword:     Nanocapsules quinine validation HPLC-DAD.


Citation:

Luana Roberta Michels, Lisiane Bajerski, Tamara Ramos Maciel, Leticia Marques Colome, Sandra Elisa Haas. Quinine-Loaded Polymeric Nanoparticles: Validation of a simple HPLC-PDA Method to Determine Drug Entrapment and Evaluation of Its Photostability. J App Pharm Sci, 2016; 6 (02): 009-015.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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