Research Article | Volume: 6, Issue: 1, January, 2016

Preparation and therapeutic evaluation of rutin-phospholipid complex for antidiabetic activity

Rashmi Vankudri,  Prasanna Habbu,  Mahesh Hiremath,  B. S. Patil,  Chetan Savant   
Rashmi Vankudri1, Prasanna Habbu2, Mahesh Hiremath2, B. S. Patil2, Chetan Savant3

1Faculty of Pharmacy, PRIST University, Thanjavur, Tamilnadu, India.

2Post graduate Department of Pharmacognosy and Phytochemistry, SET’s College of Pharmacy, Dharwad- 580 002 Karnataka, India.

3Post graduate Department of pharmacology, SET’s College of Pharmacy, Dharwad, Karnataka, India.

Open Access   

Published:  Jan 26, 2016

DOI: 10.7324/JAPS.2016.600116
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Abstract

The objective of the present work to prepare Rutin-phospholipid complex (RPC), a phytoformulation and characterization, evaluation for its antidiabetic activity in streptozotocin induced diabetic model. Prepared RPC was characterized and screened for antidiabetic activity by OGTT in normoglycemic and diabetic rats for RT and RPC at different time intervals. Effect of RT and RPC (50 and 100 mg/kg b.w. p.o. respectively) in STZ induced diabetic rats for one day and fifteen days was studied. This is followed by estimation of Estimation of SG, and lipid parameters. Histopathology studies of pancreatic tissue and bioavailability studies of RT & RPC were also carried out. SEM data showed that RPC has irregular size vesicles consisting of phosphatidylcholine. RPC showed a single endothermal peak at 147.68°C in DSC studies. OH group has shifted to lower frequency in phytosome compared to rutin phospholipid in FTIR spectra. Treatment with RPC (50 and 100 mg/kg b.w. p.o.) significantly reduced the blood glucose levels and restored the altered lipid parameters as compared to RT. Histopathological studies revealed that RPC also restored back the size of pancreatic islets and maintained the normal β-cells. A higher serum concentration of RT (13.20 µg/mL) in RPC treated group was observed in bioavailability studies as compared to RT. RPC maintained effective concentration of rutin for a longer period in rat serum.


Keyword:     Rutin phosoholipid complex antidiabetic activity OGTT bioavailability.

Citation:

Vankudri R, Habbu P, Hiremath M, Patil BS, Savant C. Preparation and therapeutic evaluation of rutin-phospholipid complex for antidiabetic activity. J App Pharm Sci, 2016; 6 (01): 090-101.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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